POU-specific domain of Oct-2 factor confers ‘octamer’ motif DNA binding specificity on heterologous Antennapedia homeodomain

• Published in: FEBS letters 1992-12, Vol.314 (3), p.361-365
• Main Authors: Brugnera, Enrico, Xu, Licen, Schaffner, Walter, Arnosti, David N.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Antennapedia Homeodomain Protein; Base Sequence; Binding and carrier proteins; Binding Sites; Biological and medical sciences; Deoxyribonuclease I; DNA - metabolism; DNA binding protein; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Homeobox; Homeodomain; Homeodomain Proteins; Methylation; Molecular Sequence Data; Nuclear Proteins; Octamer factor; Octamer Transcription Factor-2; POU domain; POU Domain Factors; Protein Binding; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Transcription Factors
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81506-H

The bipartite DNA binding domain of the POU family of transcription factors contains a ‘POU-specific’ domain unique to this class of factors and a ‘POU homeodomain’ homologous to other homeodomains. We compared DNA binding of the Oct-2 factor POU domain and the Antennapedia (Antp) homeodomain with a chimeric Oct-2/Antp protein in which the distantly related Antp homeodomain was substituted for the Oct-2 POU homeodomain. The Oct-2/Antp chimeric protein bound both the octamer and the Antp sites efficiently, indicating that DNA binding specificity is contributed by both components of the POU domain.