2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman's Reagent in Thiol-Quantification Enzyme Assays

Fluorescent sensor for thiols: Deprotection of nonfluorescent 1 by thiols (R′SH) proceeds rapidly and near‐quantitatively in aqueous solution (pH 7.4) to produce highly fluorescent 2. Assays performed in the presence of 1 provide a rapid and simple method for the determination of inhibitory constant...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2005-05, Vol.44 (19), p.2922-2925
Main Authors: Maeda, Hatsuo, Matsuno, Hiromi, Ushida, Mai, Katayama, Kohei, Saeki, Kanako, Itoh, Norio
Format: Article
Language:English
Subjects:
Butyrylcholinesterase - chemistry
Cholinesterase Inhibitors - chemistry
Dithionitrobenzoic Acid - chemistry
enzymes
Fluorescein - chemistry
Fluoresceins - chemistry
fluorescence
Fluorescent Dyes - chemistry
high-throughput screening
Humans
inhibitors
Molecular Structure
Sensitivity and Specificity
sensors
Sulfhydryl Compounds - analysis
Sulfhydryl Compounds - chemistry
Sulfones - chemistry
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Summary:Fluorescent sensor for thiols: Deprotection of nonfluorescent 1 by thiols (R′SH) proceeds rapidly and near‐quantitatively in aqueous solution (pH 7.4) to produce highly fluorescent 2. Assays performed in the presence of 1 provide a rapid and simple method for the determination of inhibitory constants for inhibitors such as donepezil toward acetyl‐ and butyrylcholinesterases.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200500114