Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Introduction: Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG...

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Bibliographic Details
Published in:Archivum Immunologiae et Therapiae Experimentalis 2009-06, Vol.57 (3), p.205-211
Main Authors: Çanakçi, Cenk Fatih, Çanakçi, Varol, Tatar, Abdulgani, Eltas, Abubekir, Sezer, Ufuk, Çiçek, Yasin, Oztas, Sitki
Format: Article
Language:English
Subjects:
8-hydroxyguanosine
Adult
Aged
Biomarkers - analysis
Biomedical and Life Sciences
Biomedicine
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - analysis
Deoxyribonucleic acid
DNA
DNA Damage
DNA, Mitochondrial - genetics
DNA, Mitochondrial - metabolism
Female
gingiva
Gingiva - metabolism
Humans
Immunology
Male
Middle Aged
Mitochondria - genetics
Mitochondrial DNA
mitochondrial DNA deletion
Oral hygiene
Original Article
oxidative DNA damage
Oxidative Stress
periodontitis
Periodontitis - etiology
Periodontitis - metabolism
Pharmacology/Toxicology
saliva
Saliva - chemistry
Sequence Deletion
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Summary:Introduction: Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG levels in saliva and mtDNA deletions in gingival tissue of patients with chronic periodontitis (CP) were evaluated. Materials and Methods: Gingival tissue and whole saliva samples were collected from 32 patients with CP and 32 healthy control subjects. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level (CAL), and probing depth (PD) were measured. Using the ELISA and polymerase chain reaction methods, the salivary 8-OHdG levels and the 7.4-kbp and 5-kbp mtDNA deletions were examined. Results: The 5-kbp mtDNA deletion was detected in 20 of the 32 periodontitis patients (62.5%), but was not detected in the healthy controls. The mean value of 8-OHdG in the saliva of the periodontitis patients with deleted mtDNA was significantly higher than in the patients with non-deleted mtDNA (p
ISSN:0004-069X
1661-4917
DOI:10.1007/s00005-009-0026-9