Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen

Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 ...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2003-07, Vol.59 (7), p.1330-1332
Main Authors: Donadini, Roberta, Wåhlberg, Malin, Kohsaka, Takao, Ito, Yasuhiko, Fields, Barry A.
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Cloning, Molecular
Crystallization
Escherichia coli - genetics
Mitogens - chemistry
Recombinant Fusion Proteins
Selenomethionine
superantigen
Superantigens - chemistry
T cell
X-Ray Diffraction
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - chemistry
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Summary:Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 Å, β = 91.97°. A native data set has been collected to a resolution of 1.8 Å using synchrotron radiation. Self‐rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444903010710