Loading…
Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen
Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 ...
Saved in:
Published in: | Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2003-07, Vol.59 (7), p.1330-1332 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c3840-2a42348d1e88107738c92febdd4c48471a9b948b6bb0a85924e93408d638bf143 |
---|---|
cites | |
container_end_page | 1332 |
container_issue | 7 |
container_start_page | 1330 |
container_title | Acta crystallographica. Section D, Biological crystallography. |
container_volume | 59 |
creator | Donadini, Roberta Wåhlberg, Malin Kohsaka, Takao Ito, Yasuhiko Fields, Barry A. |
description | Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 Å, β = 91.97°. A native data set has been collected to a resolution of 1.8 Å using synchrotron radiation. Self‐rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%. |
doi_str_mv | 10.1107/S0907444903010710 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73406085</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73406085</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3840-2a42348d1e88107738c92febdd4c48471a9b948b6bb0a85924e93408d638bf143</originalsourceid><addsrcrecordid>eNqFkEtv1DAUhS0Eog_6A7pBWbFLuX5MbC9LoC1SBYsWwSwqy4lvKhcnmdoJEH59Xc0IKrFgdV_fOdI9hBxTOKEU5Nsr0CCFEBo45JnCM7JPudYlgJDPn_R75CClOwBgjMuXZI8yxZkCtk9u6rikyYbgf9vJj0NhB1dsIgbf-8HGpfhWRrvkrQ1L8qkYu2KNMfnB22KTcHbjNDcY2zmM-Vw6jP4HuqL303iLwyvyorMh4dGuHpIvZx-u64vy8vP5x_r0smy5ElAyKxgXylFUKv8huWo167BxTrRCCUmtbrRQTdU0YNVKM4GaC1Cu4qrpqOCH5M3WdxPH-xnTZHqfWgzBDjjOychMV6BWGaRbsI1jShE7s4m-z38aCuYxU_NPplnzemc-Nz26v4pdiBlQW-CnD7j839Gcrt_Xa6Ds0bvcSn2a8NcfqY3fTSW5XJmvn85NdQX84qyW5h1_APUIkSs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73406085</pqid></control><display><type>article</type><title>Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen</title><source>Wiley-Blackwell Read & Publish Collection</source><source>Alma/SFX Local Collection</source><creator>Donadini, Roberta ; Wåhlberg, Malin ; Kohsaka, Takao ; Ito, Yasuhiko ; Fields, Barry A.</creator><creatorcontrib>Donadini, Roberta ; Wåhlberg, Malin ; Kohsaka, Takao ; Ito, Yasuhiko ; Fields, Barry A.</creatorcontrib><description>Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 Å, β = 91.97°. A native data set has been collected to a resolution of 1.8 Å using synchrotron radiation. Self‐rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.</description><identifier>ISSN: 1399-0047</identifier><identifier>ISSN: 0907-4449</identifier><identifier>EISSN: 1399-0047</identifier><identifier>DOI: 10.1107/S0907444903010710</identifier><identifier>PMID: 12832802</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: Munksgaard International Publishers</publisher><subject>Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Cloning, Molecular ; Crystallization ; Escherichia coli - genetics ; Mitogens - chemistry ; Recombinant Fusion Proteins ; Selenomethionine ; superantigen ; Superantigens - chemistry ; T cell ; X-Ray Diffraction ; Yersinia pseudotuberculosis ; Yersinia pseudotuberculosis - chemistry</subject><ispartof>Acta crystallographica. Section D, Biological crystallography., 2003-07, Vol.59 (7), p.1330-1332</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3840-2a42348d1e88107738c92febdd4c48471a9b948b6bb0a85924e93408d638bf143</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12832802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Donadini, Roberta</creatorcontrib><creatorcontrib>Wåhlberg, Malin</creatorcontrib><creatorcontrib>Kohsaka, Takao</creatorcontrib><creatorcontrib>Ito, Yasuhiko</creatorcontrib><creatorcontrib>Fields, Barry A.</creatorcontrib><title>Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen</title><title>Acta crystallographica. Section D, Biological crystallography.</title><addtitle>Acta Cryst. D</addtitle><description>Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 Å, β = 91.97°. A native data set has been collected to a resolution of 1.8 Å using synchrotron radiation. Self‐rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.</description><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Cloning, Molecular</subject><subject>Crystallization</subject><subject>Escherichia coli - genetics</subject><subject>Mitogens - chemistry</subject><subject>Recombinant Fusion Proteins</subject><subject>Selenomethionine</subject><subject>superantigen</subject><subject>Superantigens - chemistry</subject><subject>T cell</subject><subject>X-Ray Diffraction</subject><subject>Yersinia pseudotuberculosis</subject><subject>Yersinia pseudotuberculosis - chemistry</subject><issn>1399-0047</issn><issn>0907-4449</issn><issn>1399-0047</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkEtv1DAUhS0Eog_6A7pBWbFLuX5MbC9LoC1SBYsWwSwqy4lvKhcnmdoJEH59Xc0IKrFgdV_fOdI9hBxTOKEU5Nsr0CCFEBo45JnCM7JPudYlgJDPn_R75CClOwBgjMuXZI8yxZkCtk9u6rikyYbgf9vJj0NhB1dsIgbf-8HGpfhWRrvkrQ1L8qkYu2KNMfnB22KTcHbjNDcY2zmM-Vw6jP4HuqL303iLwyvyorMh4dGuHpIvZx-u64vy8vP5x_r0smy5ElAyKxgXylFUKv8huWo167BxTrRCCUmtbrRQTdU0YNVKM4GaC1Cu4qrpqOCH5M3WdxPH-xnTZHqfWgzBDjjOychMV6BWGaRbsI1jShE7s4m-z38aCuYxU_NPplnzemc-Nz26v4pdiBlQW-CnD7j839Gcrt_Xa6Ds0bvcSn2a8NcfqY3fTSW5XJmvn85NdQX84qyW5h1_APUIkSs</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Donadini, Roberta</creator><creator>Wåhlberg, Malin</creator><creator>Kohsaka, Takao</creator><creator>Ito, Yasuhiko</creator><creator>Fields, Barry A.</creator><general>Munksgaard International Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen</title><author>Donadini, Roberta ; Wåhlberg, Malin ; Kohsaka, Takao ; Ito, Yasuhiko ; Fields, Barry A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3840-2a42348d1e88107738c92febdd4c48471a9b948b6bb0a85924e93408d638bf143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Cloning, Molecular</topic><topic>Crystallization</topic><topic>Escherichia coli - genetics</topic><topic>Mitogens - chemistry</topic><topic>Recombinant Fusion Proteins</topic><topic>Selenomethionine</topic><topic>superantigen</topic><topic>Superantigens - chemistry</topic><topic>T cell</topic><topic>X-Ray Diffraction</topic><topic>Yersinia pseudotuberculosis</topic><topic>Yersinia pseudotuberculosis - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Donadini, Roberta</creatorcontrib><creatorcontrib>Wåhlberg, Malin</creatorcontrib><creatorcontrib>Kohsaka, Takao</creatorcontrib><creatorcontrib>Ito, Yasuhiko</creatorcontrib><creatorcontrib>Fields, Barry A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Donadini, Roberta</au><au>Wåhlberg, Malin</au><au>Kohsaka, Takao</au><au>Ito, Yasuhiko</au><au>Fields, Barry A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen</atitle><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle><addtitle>Acta Cryst. D</addtitle><date>2003-07</date><risdate>2003</risdate><volume>59</volume><issue>7</issue><spage>1330</spage><epage>1332</epage><pages>1330-1332</pages><issn>1399-0047</issn><issn>0907-4449</issn><eissn>1399-0047</eissn><abstract>Yersinia pseudotuberculosis‐derived mitogen (YPM), a superantigen with no amino‐acid sequence similarity to other known superantigens, has been crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group C2, with unit‐cell parameters a = 138.67, b = 78.66, c = 32.91 Å, β = 91.97°. A native data set has been collected to a resolution of 1.8 Å using synchrotron radiation. Self‐rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>Munksgaard International Publishers</pub><pmid>12832802</pmid><doi>10.1107/S0907444903010710</doi><tpages>3</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1399-0047 |
ispartof | Acta crystallographica. Section D, Biological crystallography., 2003-07, Vol.59 (7), p.1330-1332 |
issn | 1399-0047 0907-4449 1399-0047 |
language | eng |
recordid | cdi_proquest_miscellaneous_73406085 |
source | Wiley-Blackwell Read & Publish Collection; Alma/SFX Local Collection |
subjects | Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Cloning, Molecular Crystallization Escherichia coli - genetics Mitogens - chemistry Recombinant Fusion Proteins Selenomethionine superantigen Superantigens - chemistry T cell X-Ray Diffraction Yersinia pseudotuberculosis Yersinia pseudotuberculosis - chemistry |
title | Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T10%3A42%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Crystallization%20and%20preliminary%20X-ray%20analysis%20of%20Yersinia%20pseudotuberculosis-derived%20mitogen&rft.jtitle=Acta%20crystallographica.%20Section%20D,%20Biological%20crystallography.&rft.au=Donadini,%20Roberta&rft.date=2003-07&rft.volume=59&rft.issue=7&rft.spage=1330&rft.epage=1332&rft.pages=1330-1332&rft.issn=1399-0047&rft.eissn=1399-0047&rft_id=info:doi/10.1107/S0907444903010710&rft_dat=%3Cproquest_cross%3E73406085%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3840-2a42348d1e88107738c92febdd4c48471a9b948b6bb0a85924e93408d638bf143%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=73406085&rft_id=info:pmid/12832802&rfr_iscdi=true |