Structure and expression of a sugarcane gene encoding a housekeeping phosphoenolpyruvate carboxylase

A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A...

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Bibliographic Details
Published in:Plant molecular biology 1992-11, Vol.20 (4), p.663-671
Main Authors: Albert, H.A. (Hawaii Univ., Honolulu, HI (USA). Dept. of Plant Molecular Physiology), Martin, T, Sun, S.S.M
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Base Sequence
Biological and medical sciences
C4 PLANTS
DNA
EXPRESION GENICA
EXPRESSION DES GENES
FOSFENOLPIRUVATO CARBOXILASA
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
GENES
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
PHOSPHOENOLPYRUVATE CARBOXYLASE
Phosphoenolpyruvate Carboxylase - genetics
Phosphoenolpyruvate Carboxylase - metabolism
PLANTAS C4
PLANTE EN C4
Poaceae - enzymology
Poaceae - genetics
SACCHARUM
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
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Summary:A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00046451