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Slowing DNA Translocation through Nanopores Using a Solution Containing Organic Salts
One of the key challenges to nanopore DNA sequencing is to slow down DNA translocation. Here, we report that the translocation velocities of various DNA homo- and copolymers through protein pores could be significantly decreased by using electrolyte solutions containing organic salts. Using a butylm...
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| Published in: | The journal of physical chemistry. B 2009-10, Vol.113 (40), p.13332-13336 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a314t-60e0ea590fd645a461aecc1b870ec1c52b8000794016cf9b8e7bffd14935d3313 |
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| cites | cdi_FETCH-LOGICAL-a314t-60e0ea590fd645a461aecc1b870ec1c52b8000794016cf9b8e7bffd14935d3313 |
| container_end_page | 13336 |
| container_issue | 40 |
| container_start_page | 13332 |
| container_title | The journal of physical chemistry. B |
| container_volume | 113 |
| creator | de Zoysa, Ranulu Samanthi S Jayawardhana, Dilani A Zhao, Qitao Wang, Deqiang Armstrong, Daniel W Guan, Xiyun |
| description | One of the key challenges to nanopore DNA sequencing is to slow down DNA translocation. Here, we report that the translocation velocities of various DNA homo- and copolymers through protein pores could be significantly decreased by using electrolyte solutions containing organic salts. Using a butylmethylimidazolium chloride (BMIM-Cl) solution instead of the commonly used KCl solution, DNA translocation rates on the order of hundreds of microseconds per nucleotide base were achieved. The much enhanced resolution of the nanopore coupled with different event blockage amplitudes produced by different nucleotides permits the convenient differentiation between various DNA molecules. |
| doi_str_mv | 10.1021/jp9040293 |
| format | article |
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| ispartof | The journal of physical chemistry. B, 2009-10, Vol.113 (40), p.13332-13336 |
| issn | 1520-6106 1520-5207 |
| language | eng |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | B: Biophysical Chemistry Biological Transport - physiology Chlorides - chemistry DNA - chemistry DNA - metabolism Imidazolines - chemistry Lipid Bilayers - chemistry Nanostructures Organic Chemicals - chemistry Salts - chemistry |
| title | Slowing DNA Translocation through Nanopores Using a Solution Containing Organic Salts |
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