Advances in molecular methods to alter chromosomes and genome in the yeast Saccharomyces cerevisiae

A fundamental issue in biotechnology is how to breed useful strains of microorganisms for efficient production of valuable biomaterials. On-going and more recent developments in gene manipulation technologies and chromosomal and genomic modifications in particular have facilitated important contribu...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2009-10, Vol.84 (6), p.1045-1052
Main Authors: Sugiyama, Minetaka, Yamagishi, Kazuo, Kim, Yeon-Hee, Kaneko, Yoshinobu, Nishizawa, Masafumi, Harashima, Satoshi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomaterials
Biotechnology
Cell cycle
Chromosomes
Chromosomes, Fungal - genetics
Cloning
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic Engineering - methods
Genome, Fungal
Genomes
Genomics
Industrial Microbiology - methods
Life Sciences
Methods
Microbial Genetics and Genomics
Microbiology
Microorganisms
Mini-Review
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Studies
Yeast
Yeasts
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Summary:A fundamental issue in biotechnology is how to breed useful strains of microorganisms for efficient production of valuable biomaterials. On-going and more recent developments in gene manipulation technologies and chromosomal and genomic modifications in particular have facilitated important contributions in this area. “Chromosome manipulation technology” as an outgrowth of “gene manipulation technology” may provide opportunities for creating novel strains of organisms with a variety of genomic constitutions. A simple and rapid chromosome splitting technology called “PCR-mediated chromosome splitting” (PCS) that we recently developed has made it possible to manipulate chromosomes and genomes on a large scale in an industrially important microorganism, Saccharomyces cerevisiae. This paper focuses on recent advances in molecular methods for altering chromosomes and genome in S. cerevisiae featuring chromosome splitting technology. These advances in introducing large-scale genomic modifications are expected to accelerate the breeding of novel strains for biotechnological purposes, and to reveal functions of presently uncharacterized chromosomal regions in S. cerevisiae and other organisms.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-009-2144-z