Towards Profiling the Gene Expression of Tyrosinase-induced Melanogenesis in HEK293 Cells: a Functional DNA Chip Microarray and Interactomics Studies

The overexpression of a single tyrosinase gene can induce conspicuous pigmentation in nonpigmented cells. We hypothesized that some unknown tyrosinase-associated genes are simultaneously regulated by melanin synthesis. To improve understanding of melanogenesis and tyrosinase-associated functions, we...

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Bibliographic Details
Published in:Journal of biomolecular structure & dynamics 2009-12, Vol.27 (3), p.331-345
Main Authors: Cho, Ick-Hyun, Lü, Zhi-Rong, Yu, Jae-Ran, Park, Yong-Doo, Yang, Jun-Mo, Hahn, Myong-Joon, Zou, Fei
Format: Article
Language:English
Subjects:
Apoptosis
DNA microarray
Gene Expression Profiling - methods
Gene Expression Regulation, Neoplastic
Humans
Interactomics
Melanins - metabolism
Melanogenesis
Microscopy, Immunoelectron
Monophenol Monooxygenase - genetics
Monophenol Monooxygenase - metabolism
Oligonucleotide Array Sequence Analysis
RNA, Messenger - metabolism
Skin Pigmentation - genetics
Tyrosinase
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Summary:The overexpression of a single tyrosinase gene can induce conspicuous pigmentation in nonpigmented cells. We hypothesized that some unknown tyrosinase-associated genes are simultaneously regulated by melanin synthesis. To improve understanding of melanogenesis and tyrosinase-associated functions, we attempted to profile the genes that are altered during melanin production in HEK293 cells by using a functional DNA chip microarray. The candidate genes were obtained based on significance analysis of microarray (SAM) and further computational prediction via protein-protein interaction (PPI) mapping suggested that newly detected hub genes were involved in melanogenesis. PPI mapping using bioinformatic tools revealed 8 genes that formed an interaction hub. The yeast two-hybridization results suggested some candidate genes could interact with tyrosinase. The present study provides information to further understand the complex factors associated with tyrosinase- induced melanogenesis and apoptosis. The approach of combining expression data analysis and predicted protein interaction partners can help identify genes involved in pigmentation.
ISSN:0739-1102
1538-0254
DOI:10.1080/07391102.2009.10507320