Apoptosis-related changes in plasma membrane glycoconjugates of peripheral blood lymphocytes in rheumatoid arthritis

Control of apoptosis (apo) is very important for diagnosis, prognosis and treatment of rheumatoid arthritis (RA). Recently, we found that an appearance of specific cell surface GC is attributable to apo and developed lectin-induced agglutination test for apo evaluation. The aim of current study was...

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Bibliographic Details
Published in:Autoimmunity (Chur, Switzerland) Switzerland), 2009-01, Vol.42 (4), p.334-336
Main Authors: Bilyy, R., Nemesh, L., Antonyuk, V., Kit, Yu, Valchuk, I., Havryluk, A., Chopyak, V., Stoika, R.
Format: Article
Language:English
Subjects:
Agglutination Tests - methods
Apoptosis
Apoptosis - immunology
Arthritis, Rheumatoid - diagnosis
Arthritis, Rheumatoid - immunology
Arthritis, Rheumatoid - pathology
Cell Membrane - immunology
Cell Membrane - metabolism
Flow Cytometry
glycoconjugates
Glycoconjugates - immunology
Glycoconjugates - metabolism
Humans
Lectins
Lymphocytes - immunology
Lymphocytes - metabolism
Lymphocytes - pathology
plasma membrane
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Summary:Control of apoptosis (apo) is very important for diagnosis, prognosis and treatment of rheumatoid arthritis (RA). Recently, we found that an appearance of specific cell surface GC is attributable to apo and developed lectin-induced agglutination test for apo evaluation. The aim of current study was to estimate changes in surface GC expression in peripheral blood lymphocytes (PBL) of normal healthy donors (NHD) and patients with RA, measured by cell agglutination with the galactose-specific VAA lectin and by lectin-cytochemical analysis. In parallel, these changes in apo incidence were evaluated by the detection of cells with sub-G1 DNA content. The data revealed an increased level of apo in lymphocytes of RA patients (n = 29), and increased cell surface GC expression in lymphocytes of NHD (n = 18). A correlation (R = 0.708) was observed between these two indicators. Specific changes in cell surface GC can be effectively used for the detection of apo cells in RA and other autoimmune disorders.
ISSN:0891-6934
1607-842X
DOI:10.1080/08916930902830979