Glutamate dehydrogenase activity in lymphocytes of B-cell chronic lymphocytic leukaemia patients

• Published in: Clinical biochemistry 2009-11, Vol.42 (16), p.1677-1684
• Main Authors: Pajič, Tadej, Černelč, Peter, Sešek Briški, Alenka, Lejko-Zupanc, Tatjana, Malešič, Ivan
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Case-Control Studies; Chronic lymphocytic leukaemia; Female; GLUD1 and GLUD2 gene expressions; Glutamate dehydrogenase; Glutamate Dehydrogenase - blood; Glutamate Dehydrogenase - genetics; Humans; Leukemia, Lymphocytic, Chronic, B-Cell - blood; Leukemia, Lymphocytic, Chronic, B-Cell - enzymology; Lymphocytes - enzymology; Male; Middle Aged; Polymerase Chain Reaction
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2009.08.003

Objectives: To investigate the pattern of glutamate dehydrogenase (GLDH) activity, GLUD1 and GLUD2 expressions in peripheral blood mononuclear cells (PBMC) of untreated B-chronic lymphocytic leukemia (B-CLL) in healthy individuals (HI) and patients with infectious mononucleosis (IM). Design and methods: GLDH activity was determined in a supernatant obtained from pelleted PBMC. GLUD1 and GLUD2 mRNA expression was determined using a quantitative real-time polymerase chain reaction. CD19 + B cells from PBMC were purified by using positive selection. Results: The highest GLDH activity was found in PBMC of the B-CLL group followed by the HI group and IM group. The PBMC GLDH activity was higher in 60% of the B-CLL patients according to the established reference interval for our HI (2.17–5.70 μkat/g protein). The greater GLDH activity was also found in the CD19 + cell preparation of the B-CLL patients (two of the three) but not in HI ( n = 3). The median value of GLUD1 expression was highest in the IM group ( n = 11), followed by the HI ( n = 14) and B-CLL groups ( n = 59) (median 4.69/3.78, P < 0.005 and 4.69/2.91, P < 0.0005, respectively). GLUD2 expression was not significantly different between groups. Conclusions: The increased GLDH activity is specific for the PBMC of B-CLL patients. The GLUD1 but not the GLUD2 gene expression pattern is different between the PBMC of IM and B-CLL patients.