An interference study of the copper-induced plasma oxidizability test

The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test. The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group...

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Bibliographic Details
Published in:Clinical biochemistry 2009-11, Vol.42 (16), p.1728-1731
Main Authors: Eskiocak, Sevgi, Ege, Turan, Yapar, Suleyman Bedir, Kaya, Sabriye, Sayilan, Gulben
Format: Article
Language:English
Subjects:
Adult
Chloride ions
Conjugated diene
Copper - pharmacology
Copper-induced lipid peroxidation
Cristaloid cardioplegic fluid
Humans
Lipid Peroxidation - drug effects
Male
Thiobarbituric Acid Reactive Substances - metabolism
Thiobarbituric acid-reactive substances
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Summary:The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test. The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group of the plasma pool. The accumulation of conjugated diene (CD) by copper-induced oxidation was monitored for a period of 5 h. Thiobarbituric acid-reactive substances (TBARS) formed during the incubation of plasma with copper was also measured. It was found that, the production of CD and TBARS were inhibited and the lag time had increased, when the plasma was mixed with CCF or its contents. As a result, we conclude that that the susceptibility of plasma to copper-induced lipid peroxidation is interfered by CCF. The chloride ions, which major content of CCF, may play an important role on this effect.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2009.08.009