Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela

Abstract The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA™ Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patient...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2009-11, Vol.65 (3), p.247-253
Main Authors: Ramirez, Alvaro H, Moros, Zoila, Comach, Guillermo, Zambrano, Julio, Bravo, Lola, Pinto, Belqys, Vielma, Silvana, Cardier, José, Liprandi, Ferdinando
Format: Article
Language:English
Subjects:
Acute Disease
Aedes - virology
Animals
Antibodies, Viral - analysis
Antibodies, Viral - blood
Antigens, Viral - analysis
Antigens, Viral - immunology
Cell Line
Chi-Square Distribution
Dengue
Dengue - blood
Dengue - immunology
Dengue - virology
Dengue virus
Dengue Virus - immunology
Dengue Virus - isolation & purification
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Humans
Infectious Disease
Internal Medicine
NS1
Sensitivity and Specificity
Venezuela
Viral Nonstructural Proteins - analysis
Viral Nonstructural Proteins - immunology
Viremia - blood
Viremia - immunology
Viremia - virology
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Summary:Abstract The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA™ Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patients, as well as 36 sera from patients with other acute febrile illnesses. PLATELIA™ was more sensitive and slightly less specific than Dengue Early ELISA (sensitivity, 71.3% versus 60.9%; specificity, 86.1% versus 94.3%, respectively). The strip test showed an overall sensitivity of 67.8% with a specificity of 94.4%. A lower sensitivity was observed with Dengue Early ELISA for dengue virus (DENV) type 4 (30%) and by the 3 tests for DENV type 2 (56.5%). The use of these kits allows for rapid and specific early diagnosis of dengue infection; however, their sensitivity for each serotype must be further evaluated to guarantee an accurate diagnosis, particularly in those regions where the 4 dengue serotypes are cocirculating.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2009.07.022