Control of PHERES1 Imprinting in Arabidopsis by Direct Tandem Repeats

Genomic imprinting is an epigenetic phenomenon that causes monoallelic expression of specific genes dependent on the parent-of-origin. Imprinting of the Arabidopsis gene PHERES1 requires the function of the FERTILIZATION INDEPENDENT SEED (FIS) Polycomb group complex as well as a distally located met...

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Bibliographic Details
Published in:Molecular plant 2009-07, Vol.2 (4), p.654-660
Main Authors: Villar, Corina Belle R., Erilova, Aleksandra, Makarevich, Grigory, Trösch, Raphael, Köhler, Claudia
Format: Article
Language:English
Subjects:
Alleles
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Chromatin Assembly and Disassembly - genetics
Chromatin Immunoprecipitation
Chromatin structure
Epigenesis, Genetic - genetics
epigenetics
gametogenesis and fertilization
Gene Expression Regulation, Plant - genetics
gene regulation
gene silencing
Genomic Imprinting - genetics
MADS Domain Proteins - genetics
Plants, Genetically Modified - genetics
Polymerase Chain Reaction
remodeling
Tandem Repeat Sequences - genetics
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Summary:Genomic imprinting is an epigenetic phenomenon that causes monoallelic expression of specific genes dependent on the parent-of-origin. Imprinting of the Arabidopsis gene PHERES1 requires the function of the FERTILIZATION INDEPENDENT SEED (FIS) Polycomb group complex as well as a distally located methylated region containing a tandem triple repeat sequence. In this study, we investigated the regulation of the close PHERES1 homolog PHERES2. We found that PHERES2 is also a direct target gene of the FIS Polycomb group complex, but, in contrast to PHERES1, PHERES2 is equally expressed from maternal and paternal alleles. Thus, PHERES2 is not regulated by genomic imprinting, correlating with the lack of tandem repeats at PHERES2. Eliminating tandem repeats from the PHERES1 locus abolishes PHERES1 imprinting, demonstrating that tandem repeats are essential for PHERES1 imprinting. Taking these results together, our study shows that the recently duplicated genes PHERES1 and PHERES2 are both target genes of the FIS Polycomb group complex but only PHERES1 is regulated by genomic imprinting, which is likely caused by the presence of repeat sequences in the proximity of the PHERES1 locus.
ISSN:1674-2052
1752-9867
DOI:10.1093/mp/ssp014