Successive bouts of cycling stimulates genes associated with mitochondrial biogenesis

Exercise increases mRNA for genes involved in mitochondrial biogenesis and oxidative enzyme capacity. However, little is known about how these genes respond to consecutive bouts of prolonged exercise. We examined the effects of 3 h of intensive cycling performed on three consecutive days on the mRNA...

Full description

Saved in:
Bibliographic Details
Published in:European journal of applied physiology 2009-11, Vol.107 (4), p.419-427
Main Authors: Dumke, Charles L., Mark Davis, J., Angela Murphy, E., Nieman, David C., Carmichael, Martin D., Quindry, John C., Travis Triplett, N., Utter, Alan C., Gross Gowin, Sarah J., Henson, Dru A., McAnulty, Steven R., McAnulty, Lisa S.
Format: Article
Language:English
Subjects:
Adult
Bicycling - physiology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Citrate (si)-Synthase - genetics
Cytochromes c - genetics
Exercise - physiology
Fundamental and applied biological sciences. Psychology
Genes, Mitochondrial
Human Physiology
Humans
Male
Mitochondria, Muscle - genetics
Mitochondria, Muscle - metabolism
Mitochondria, Muscle - physiology
Mitochondrial Proteins - genetics
Occupational Medicine/Industrial Medicine
Original Article
Periodicity
Physical Endurance - genetics
Physical Endurance - physiology
RNA, Messenger - metabolism
Sirtuin 1 - genetics
Sports Medicine
Up-Regulation - genetics
Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Exercise increases mRNA for genes involved in mitochondrial biogenesis and oxidative enzyme capacity. However, little is known about how these genes respond to consecutive bouts of prolonged exercise. We examined the effects of 3 h of intensive cycling performed on three consecutive days on the mRNA associated with mitochondrial biogenesis in trained human subjects. Forty trained cyclists were tested for VO 2max (54.7 ± 1.1 ml kg −1  min −1 ). The subjects cycled at 57% watts max for 3 h using their own bicycles on CompuTrainer™ Pro Model trainers (RacerMate, Seattle, WA) on three consecutive days. Muscle biopsies were obtained from the vastus lateralis pre- and post-exercise on days one and three. Muscle samples were analyzed for mRNA content of peroxisome proliferator receptor gamma coactivator-1 alpha (PGC-1α), sirtuin 1 (Sirt-1), cytochrome c , and citrate synthase. Data were analyzed using a 2 (time) × 2 (day) repeated measures ANOVA. Of the mRNA analyzed, the following increased from pre to post 3 h rides: cytochrome c ( P  = 0.006), citrate synthase ( P  = 0.03), PGC-1α ( P  
ISSN:1439-6319
1439-6327
DOI:10.1007/s00421-009-1143-1