First isolation and characterization of Lactococcus garvieae from Brazilian Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz)

Lactococcus garvieae infection in cultured Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz), from Brazil is reported. The commercial bacterial identification system, Biolog Microlog®, confirmed the identity of L. garvieae. Infectivity trials co...

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Bibliographic Details
Published in:Journal of fish diseases 2009-11, Vol.32 (11), p.943-951
Main Authors: Evans, J.J, Klesius, P.H, Shoemaker, C.A
Format: Article
Language:English
Subjects:
analytical kits
animal pathogenic bacteria
Animals
bacterial infections
Bacterial Typing Techniques - veterinary
Biolog Microlog
Brazil
Catfishes - microbiology
Cichlids - microbiology
fish culture
fish diseases
Fish Diseases - microbiology
Fish Diseases - mortality
Fishes
freshwater fish
Gram-Positive Bacterial Infections - microbiology
Gram-Positive Bacterial Infections - mortality
Gram-Positive Bacterial Infections - veterinary
Lactococcus - classification
Lactococcus - isolation & purification
Lactococcus - metabolism
Lactococcus - pathogenicity
Lactococcus - physiology
Lactococcus garvieae
Lactococcus lactis
new geographic records
Oreochromis niloticus
pathogen identification
pintado
Pseudoplathystoma corruscans
tilapia
tilapia (common name)
Time Factors
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Summary:Lactococcus garvieae infection in cultured Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz), from Brazil is reported. The commercial bacterial identification system, Biolog Microlog®, confirmed the identity of L. garvieae. Infectivity trials conducted in Nile tilapia using Brazilian Nile tilapia L. garvieae isolates resulted in a median lethal dose-50 of 1.4 x 10⁵ colony-forming units (CFU)/fish. This is the first evidence of the presence of this pathogen from Brazilian fish. In addition, this is the first report of L. garvieae infection in either Nile tilapia or pintado. Collectively, this evidence expands the geographical range of fish hosts, number of fish hosts harbouring L. garvieae and carbon source utilization by L. garvieae fish isolates. Furthermore, the Biolog system may be an alternative technique to polymerase chain reaction for the identification of L. garvieae and discrimination between closely related bacterial species.
ISSN:0140-7775
1365-2761
DOI:10.1111/j.1365-2761.2009.01075.x