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Cisplatin and Oxaliplatin Cytotoxic Effects in Sensitive and Cisplatin-resistant Human Cervical Tumor Cells: Time and Mode of Application Dependency
Background: Time-dependence of cisplatin (CDDP) and oxaliplatin (L-OHP) cytotoxic effects in A431 and A431/Pt cells (sensitive and CDDP-resistant human cervical tumor cells) were investigated. Materials and Methods: The drug application modes were pulse (12.5, 25 or 50 μM up to 72 h) and pulse-plus...
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| Published in: | Anticancer research 2009-10, Vol.29 (10), p.3931-3937 |
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| container_end_page | 3937 |
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| container_title | Anticancer research |
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| creator | MARTELLI, Laura RAGAZZI, Eugenio DI MARIO, Francesco BASATO, Marino MARTELLI, Mario |
| description | Background: Time-dependence of cisplatin (CDDP) and oxaliplatin (L-OHP) cytotoxic effects in A431 and A431/Pt cells (sensitive
and CDDP-resistant human cervical tumor cells) were investigated. Materials and Methods: The drug application modes were pulse
(12.5, 25 or 50 μM up to 72 h) and pulse-plus-chase (50 μM for 2, 4 or 6h, followed by washing and 72 h-incubation in drug-free
medium). Results: In the A431 cells, the pulse drug application showed time-effect curves with two plateaux; the inhibitory
activity of CDDP was higher than that of L-OHP. The same growth-inhibition fraction was reached by L-OHP in a longer time
than CDDP. In the A431/Pt cells, the curve shapes for both drugs were similar in both application modes and had the same general
characteristics, noted in the parental cell line. CDDP appeared less active than L-OHP. Conclusion: Different cytotoxicity
curves of Pt-drugs could be dictated by the presence of the bulky diaminocyclohexane (DACH) ligand, affecting the kinetics
of Pt-DNA binding; mismatch repair (MSH2) protein is involved in the resistance. |
| format | article |
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and CDDP-resistant human cervical tumor cells) were investigated. Materials and Methods: The drug application modes were pulse
(12.5, 25 or 50 μM up to 72 h) and pulse-plus-chase (50 μM for 2, 4 or 6h, followed by washing and 72 h-incubation in drug-free
medium). Results: In the A431 cells, the pulse drug application showed time-effect curves with two plateaux; the inhibitory
activity of CDDP was higher than that of L-OHP. The same growth-inhibition fraction was reached by L-OHP in a longer time
than CDDP. In the A431/Pt cells, the curve shapes for both drugs were similar in both application modes and had the same general
characteristics, noted in the parental cell line. CDDP appeared less active than L-OHP. Conclusion: Different cytotoxicity
curves of Pt-drugs could be dictated by the presence of the bulky diaminocyclohexane (DACH) ligand, affecting the kinetics
of Pt-DNA binding; mismatch repair (MSH2) protein is involved in the resistance.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 19846931</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - pharmacokinetics ; Biological and medical sciences ; Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - metabolism ; Cell Line, Tumor ; Cisplatin - administration & dosage ; Cisplatin - pharmacokinetics ; Dose-Response Relationship, Drug ; Drug Resistance, Neoplasm ; Drug Screening Assays, Antitumor ; Female ; Humans ; Medical sciences ; Organoplatinum Compounds - administration & dosage ; Organoplatinum Compounds - pharmacokinetics ; Structure-Activity Relationship ; Tumors ; Uterine Cervical Neoplasms - drug therapy ; Uterine Cervical Neoplasms - metabolism</subject><ispartof>Anticancer research, 2009-10, Vol.29 (10), p.3931-3937</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22076894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19846931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MARTELLI, Laura</creatorcontrib><creatorcontrib>RAGAZZI, Eugenio</creatorcontrib><creatorcontrib>DI MARIO, Francesco</creatorcontrib><creatorcontrib>BASATO, Marino</creatorcontrib><creatorcontrib>MARTELLI, Mario</creatorcontrib><title>Cisplatin and Oxaliplatin Cytotoxic Effects in Sensitive and Cisplatin-resistant Human Cervical Tumor Cells: Time and Mode of Application Dependency</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Time-dependence of cisplatin (CDDP) and oxaliplatin (L-OHP) cytotoxic effects in A431 and A431/Pt cells (sensitive
and CDDP-resistant human cervical tumor cells) were investigated. Materials and Methods: The drug application modes were pulse
(12.5, 25 or 50 μM up to 72 h) and pulse-plus-chase (50 μM for 2, 4 or 6h, followed by washing and 72 h-incubation in drug-free
medium). Results: In the A431 cells, the pulse drug application showed time-effect curves with two plateaux; the inhibitory
activity of CDDP was higher than that of L-OHP. The same growth-inhibition fraction was reached by L-OHP in a longer time
than CDDP. In the A431/Pt cells, the curve shapes for both drugs were similar in both application modes and had the same general
characteristics, noted in the parental cell line. CDDP appeared less active than L-OHP. Conclusion: Different cytotoxicity
curves of Pt-drugs could be dictated by the presence of the bulky diaminocyclohexane (DACH) ligand, affecting the kinetics
of Pt-DNA binding; mismatch repair (MSH2) protein is involved in the resistance.</description><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - drug therapy</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cisplatin - administration & dosage</subject><subject>Cisplatin - pharmacokinetics</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Resistance, Neoplasm</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Female</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Organoplatinum Compounds - administration & dosage</subject><subject>Organoplatinum Compounds - pharmacokinetics</subject><subject>Structure-Activity Relationship</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - drug therapy</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNo90N9u2yAUBnCrWtWkaV9h4mbblSX-2AZ2V2XdWqlTL5peW9gcFiaMPcBp8x594JEl6xU66Pd94nBWLAmXpOQ1wx-KJaY1LjnG9aK4jPE3xk0jBbsoFkSKqpGMLIu3tY2TU8l6pLxGj6_K2dO83qcxja-2R7fGQJ8iypdP4KNNdgf_-Hu4DBBtTMondDcPKoch7GyvHNrMwxjy6Fz8ijZ2OAZ_jhrQaNDNNLnMkh09-gYTeA2-318V50a5CNenc1U8f7_drO_Kh8cf9-ubh3JLOUmlqLQ0VHedIJrxBgQVrJacdYpg02FiOKVUa1kL3nVVBcIYxjsBUPW4MkKyVfHl2DuF8c8MMbWDjX1-qvIwzrHlrMKyls1BfjzJuRtAt1Owgwr79v9HZvDpBFTMa5ugfG_ju6MU80bIKrvPR7e1v7YvNkAbB-VcrmWtClS2BLfsUPgX3qGMKQ</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>MARTELLI, Laura</creator><creator>RAGAZZI, Eugenio</creator><creator>DI MARIO, Francesco</creator><creator>BASATO, Marino</creator><creator>MARTELLI, Mario</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Cisplatin and Oxaliplatin Cytotoxic Effects in Sensitive and Cisplatin-resistant Human Cervical Tumor Cells: Time and Mode of Application Dependency</title><author>MARTELLI, Laura ; RAGAZZI, Eugenio ; DI MARIO, Francesco ; BASATO, Marino ; MARTELLI, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h271t-84d9f2dbb81d376e82835973ba10fb01f7222dd9587bb44e8ff37b8ee4c04f893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - drug therapy</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cisplatin - administration & dosage</topic><topic>Cisplatin - pharmacokinetics</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Resistance, Neoplasm</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Female</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Organoplatinum Compounds - administration & dosage</topic><topic>Organoplatinum Compounds - pharmacokinetics</topic><topic>Structure-Activity Relationship</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - drug therapy</topic><topic>Uterine Cervical Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MARTELLI, Laura</creatorcontrib><creatorcontrib>RAGAZZI, Eugenio</creatorcontrib><creatorcontrib>DI MARIO, Francesco</creatorcontrib><creatorcontrib>BASATO, Marino</creatorcontrib><creatorcontrib>MARTELLI, Mario</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MARTELLI, Laura</au><au>RAGAZZI, Eugenio</au><au>DI MARIO, Francesco</au><au>BASATO, Marino</au><au>MARTELLI, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cisplatin and Oxaliplatin Cytotoxic Effects in Sensitive and Cisplatin-resistant Human Cervical Tumor Cells: Time and Mode of Application Dependency</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>29</volume><issue>10</issue><spage>3931</spage><epage>3937</epage><pages>3931-3937</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Time-dependence of cisplatin (CDDP) and oxaliplatin (L-OHP) cytotoxic effects in A431 and A431/Pt cells (sensitive
and CDDP-resistant human cervical tumor cells) were investigated. Materials and Methods: The drug application modes were pulse
(12.5, 25 or 50 μM up to 72 h) and pulse-plus-chase (50 μM for 2, 4 or 6h, followed by washing and 72 h-incubation in drug-free
medium). Results: In the A431 cells, the pulse drug application showed time-effect curves with two plateaux; the inhibitory
activity of CDDP was higher than that of L-OHP. The same growth-inhibition fraction was reached by L-OHP in a longer time
than CDDP. In the A431/Pt cells, the curve shapes for both drugs were similar in both application modes and had the same general
characteristics, noted in the parental cell line. CDDP appeared less active than L-OHP. Conclusion: Different cytotoxicity
curves of Pt-drugs could be dictated by the presence of the bulky diaminocyclohexane (DACH) ligand, affecting the kinetics
of Pt-DNA binding; mismatch repair (MSH2) protein is involved in the resistance.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>19846931</pmid><tpages>7</tpages></addata></record> |
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| subjects | Antineoplastic Agents - administration & dosage Antineoplastic Agents - pharmacokinetics Biological and medical sciences Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - metabolism Cell Line, Tumor Cisplatin - administration & dosage Cisplatin - pharmacokinetics Dose-Response Relationship, Drug Drug Resistance, Neoplasm Drug Screening Assays, Antitumor Female Humans Medical sciences Organoplatinum Compounds - administration & dosage Organoplatinum Compounds - pharmacokinetics Structure-Activity Relationship Tumors Uterine Cervical Neoplasms - drug therapy Uterine Cervical Neoplasms - metabolism |
| title | Cisplatin and Oxaliplatin Cytotoxic Effects in Sensitive and Cisplatin-resistant Human Cervical Tumor Cells: Time and Mode of Application Dependency |
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