Molecular Imaging Application of Radioiodinated Anti-EGFR Human Fab to EGFR-overexpressing Tumor Xenografts

Background: Suitable diagnostics could identify patients who might benefit from targeted therapies. Molecular imaging is a promising method estimating the expression of specific molecules in vivo, and the goal of this study was to evaluate a radioiodinated anti-epidermal growth factor receptor (EGFR...

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Bibliographic Details
Published in:Anticancer research 2009-10, Vol.29 (10), p.4005-4011
Main Authors: Xu, Ning, Cai, Gangming, Ye, Wanzhong, Wang, Xin, Li, Yunqian, Zhao, Ping, Zhang, Aixia, Zhang, Rongjun, Cao, Brian
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line, Tumor
Female
Humans
Immunoconjugates - chemistry
Immunoglobulin Fragments - chemistry
Immunoglobulin Fragments - immunology
Immunoprecipitation
Iodine Radioisotopes - chemistry
Isotope Labeling
Medical sciences
Mice
Mice, Nude
Neoplasms - diagnostic imaging
Neoplasms - enzymology
NIH 3T3 Cells
Radiopharmaceuticals - chemical synthesis
Receptor, Epidermal Growth Factor - biosynthesis
Receptor, Epidermal Growth Factor - immunology
Tomography, Emission-Computed, Single-Photon
Transplantation, Heterologous
Tumors
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Summary:Background: Suitable diagnostics could identify patients who might benefit from targeted therapies. Molecular imaging is a promising method estimating the expression of specific molecules in vivo, and the goal of this study was to evaluate a radioiodinated anti-epidermal growth factor receptor (EGFR) human Fab as a molecular imaging agent for diagnosis. Materials and Methods: Three human tumor cell lines representing tumors with different levels of EGFR expression were selected and their corresponding xenografts produced. 125 I was conjugated to a human anti-EGFR Fab that recognizes the native extracellular domain of EGFR evidenced by immunoprecipitation (IP) and fluorescence-activated cell sorting (FACS) assays. Single-photon-emission computed tomography (SPECT) imaging of 125 I-Fab being administered to nude mice bearing xenografts were obtained, and further analyzed by region of interest (ROI) assay. Results: The 125 I-Fab was achieved successfully without losing its immunoreactivity. The scintigrams as well as ROI assay showed that 125 I-Fab was able to clearly quantitatively distinguish the different expression levels of EGFR in vivo. Conclusion: 125 I-Fab is a potential molecular imaging agent for clinical diagnosis of EGFR-overexpressing tumors.
ISSN:0250-7005
1791-7530