Macrolide resistance determinants of group A streptococci in Ankara, Turkey

Objectives: Macrolide–lincosamide–streptogramin B (MLSB) resistance determinants of group A streptococci (GAS) in Ankara, Turkey, were defined for the first time. Isolates and methods: A total of 1355 GAS isolates, collected from three different regions of Ankara, were screened for erythromycin resi...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2003-07, Vol.52 (1), p.110-112
Main Authors: Acikgoz, Ziya Cibali, Gocer, Safiye, Tuncer, Serdar
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Drug Resistance, Bacterial
ermA gene
Erythromycin - pharmacology
Genotype
Humans
macrolide–lincosamide–streptogramin B resistance
Medical sciences
Microbial Sensitivity Tests
MLSB
Pharmacology. Drug treatments
Phenotype
Streptococcal Infections - microbiology
Streptococcus
Streptococcus pyogenes
Streptococcus pyogenes - drug effects
Streptococcus pyogenes - genetics
Turkey
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Summary:Objectives: Macrolide–lincosamide–streptogramin B (MLSB) resistance determinants of group A streptococci (GAS) in Ankara, Turkey, were defined for the first time. Isolates and methods: A total of 1355 GAS isolates, collected from three different regions of Ankara, were screened for erythromycin resistance. Resistance phenotypes were determined by a triple-disc test, and the gene determinants responsible were determined by PCR. MICs of erythromycin, clindamycin and spiramycin were measured for the resistant isolates, and susceptibility rates to some further antibiotics were determined. Results: Thirty-six isolates (2.6%) were resistant to erythromycin. Of these, 17 (47.2%) expressed macrolide-restricted resistance (M phenotype), while the remainder expressed inducible (16 isolates, 44.4%) or constitutive (three isolates, 8.3%) MLSB resistance. All isolates of the M phenotype harboured the mef(A) gene. Of non-M isolates, 14 harboured erm(A) subclass erm(TR) and five had erm(B) genes. There was a significant relationship between tetracycline resistance and the inducible phenotype (P < 0.05). Macrolide resistance was significantly higher in adults (P < 0.05), and increased more than two-fold in 2002 compared with 2001 (P < 0.05). Conclusion: The prevalence of macrolide resistance in GAS is low in Ankara; therefore, routine antimicrobial susceptibility testing against these agents seems unwarranted.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/dkg300