Insulin‐like growth factor 1 receptor expression in wild‐type GISTs: A potential novel therapeutic target

Aberrations of the Insulin‐like Growth Factor (IGF) system have been found in association with a variety of cancer types. The potential role of IGF1R has been postulated in a small subset of GISTs, but until now the implications of its aberrations have not been defined. The aim of the study was to e...

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Bibliographic Details
Published in:International journal of cancer 2009-12, Vol.125 (12), p.2991-2994
Main Authors: Pantaleo, Maria A., Astolfi, Annalisa, Di Battista, Monica, Heinrich, Michael C., Paterini, Paola, Scotlandi, Katia, Santini, Donatella, Catena, Fausto, Manara, Maria C., Nannini, Margherita, Maleddu, Alessandra, Saponara, Maristella, Lolli, Cristian, Formica, Serena, Biasco, Guido
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Blotting, Western
Gastrointestinal Stromal Tumors - genetics
Gastrointestinal Stromal Tumors - metabolism
Gastrointestinal Stromal Tumors - secondary
gastrointestinal stromal tumours
Gene Amplification
Gene Expression Profiling
Humans
Immunoenzyme Techniques
insulin‐like growth factor 1 receptor
KIT receptor
Medical sciences
Middle Aged
Mutation - genetics
Oligonucleotide Array Sequence Analysis
PDGFRA
Polymorphism, Single Nucleotide - genetics
Proto-Oncogene Proteins c-kit - metabolism
Receptor, IGF Type 1 - genetics
Receptor, IGF Type 1 - metabolism
Receptor, Platelet-Derived Growth Factor alpha - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tumors
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Summary:Aberrations of the Insulin‐like Growth Factor (IGF) system have been found in association with a variety of cancer types. The potential role of IGF1R has been postulated in a small subset of GISTs, but until now the implications of its aberrations have not been defined. The aim of the study was to examine the IGF1R status in patients with gastric GIST in regard to KIT and PDGFRA genotype. Fresh resection specimens were collected from 8 primary tumours [2 wild‐type (WT) and 6 mutant cases]. IGF1R was studied as gene expression profiling with Affymetrix GeneChip HG‐U133Plus 2.0 arrays and as genomic copy number with SNP array analysis Affymetrix Genome Wide Human SNP 6.0 arrays, and at protein level with western blotting (WB) and immunohistochemistry (IHC). The unsupervised analysis of gene expression profiling of our patients merged with a data set from gastric GISTs identified 2 patients out of 8 with different expression of IGF1R. The data were confirmed by WB and IHC. In particular, IGF1R was upregulated in 2 young patients (
ISSN:0020-7136
1097-0215
1097-0215
DOI:10.1002/ijc.24595