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A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro
Purpose Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile pros...
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| Published in: | Archives of gynecology and obstetrics 2009-12, Vol.280 (6), p.961-969 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273 |
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| cites | cdi_FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273 |
| container_end_page | 969 |
| container_issue | 6 |
| container_start_page | 961 |
| container_title | Archives of gynecology and obstetrics |
| container_volume | 280 |
| creator | Baston-Büst, Dunja M. Hess, Alexandra P. Hirchenhain, Jens Krücken, Jürgen Wunderlich, Frank Krüssel, Jan S. Friebe-Hoffmann, Ulrike K. |
| description | Purpose
Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE
2
in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.
Methods
In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.
Results
We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.
Conclusions
These effects might potentially contribute to birth-associated induction of contractions in vivo. |
| doi_str_mv | 10.1007/s00404-009-1046-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_734103741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>734103741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMo7rr6A7xIwYOn6kySNu1xEb9gwYt6DWmSapd-rEm7uP_elC4sCEIgGfLMO8NDyCXCLQKIOw_AgccAeYzA0zg7InPkjMYgEI_JHPLxDamYkTPv1wBIsyw9JTPMGeZJgnPysYw2nfdVUdtINUW1VbVt-8h1oS47Fzlbq5-qjcL5GhrVRs2ua2zvKlVHqjWRsboyQyi0rWs_Ytuqd905OSlV7e3F_l6Q98eHt_vnePX69HK_XMWaCexjkegs7AIpFKxIlBKCM1OyXGlgqHJkRhtNgbMyN7pAqxRSXpTMWKHykgq2IDdT7sZ134P1vWwqP66iWtsNXgrGEZjgGMjrP-S6G1wblpOUpphlkFEeKJwo7YIVZ0u5cVWj3E4iyNG5nJzL4FyOzmUWeq72yUPRWHPo2EsOAJ0AH77aT-sOo_9P_QX6EYvD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2261880824</pqid></control><display><type>article</type><title>A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro</title><source>Springer Link</source><creator>Baston-Büst, Dunja M. ; Hess, Alexandra P. ; Hirchenhain, Jens ; Krücken, Jürgen ; Wunderlich, Frank ; Krüssel, Jan S. ; Friebe-Hoffmann, Ulrike K.</creator><creatorcontrib>Baston-Büst, Dunja M. ; Hess, Alexandra P. ; Hirchenhain, Jens ; Krücken, Jürgen ; Wunderlich, Frank ; Krüssel, Jan S. ; Friebe-Hoffmann, Ulrike K.</creatorcontrib><description>Purpose
Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE
2
in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.
Methods
In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.
Results
We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.
Conclusions
These effects might potentially contribute to birth-associated induction of contractions in vivo.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-009-1046-8</identifier><identifier>PMID: 19319551</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Adult ; Blotting, Western ; Cyclooxygenase 1 - biosynthesis ; Cyclooxygenase 1 - genetics ; Cyclooxygenase 1 - metabolism ; Cyclooxygenase 2 - biosynthesis ; Cyclooxygenase 2 - genetics ; Cyclooxygenase 2 - metabolism ; Decidua - cytology ; Decidua - drug effects ; Decidua - enzymology ; Decidua - physiology ; Dinoprostone - metabolism ; Endocrinology ; Enzyme Activation - drug effects ; Enzyme-Linked Immunosorbent Assay ; Female ; Gynecology ; Human Genetics ; Humans ; Immunohistochemistry ; Medicine ; Medicine & Public Health ; Myometrium - drug effects ; Myometrium - enzymology ; Myometrium - physiology ; Obstetric Labor, Premature - prevention & control ; Obstetrics/Perinatology/Midwifery ; Original Article ; Pregnancy ; Relaxin - pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Uterine Contraction - drug effects ; Young Adult</subject><ispartof>Archives of gynecology and obstetrics, 2009-12, Vol.280 (6), p.961-969</ispartof><rights>Springer-Verlag 2009</rights><rights>Archives of Gynecology and Obstetrics is a copyright of Springer, (2009). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273</citedby><cites>FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19319551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baston-Büst, Dunja M.</creatorcontrib><creatorcontrib>Hess, Alexandra P.</creatorcontrib><creatorcontrib>Hirchenhain, Jens</creatorcontrib><creatorcontrib>Krücken, Jürgen</creatorcontrib><creatorcontrib>Wunderlich, Frank</creatorcontrib><creatorcontrib>Krüssel, Jan S.</creatorcontrib><creatorcontrib>Friebe-Hoffmann, Ulrike K.</creatorcontrib><title>A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE
2
in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.
Methods
In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.
Results
We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.
Conclusions
These effects might potentially contribute to birth-associated induction of contractions in vivo.</description><subject>Adult</subject><subject>Blotting, Western</subject><subject>Cyclooxygenase 1 - biosynthesis</subject><subject>Cyclooxygenase 1 - genetics</subject><subject>Cyclooxygenase 1 - metabolism</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Decidua - cytology</subject><subject>Decidua - drug effects</subject><subject>Decidua - enzymology</subject><subject>Decidua - physiology</subject><subject>Dinoprostone - metabolism</subject><subject>Endocrinology</subject><subject>Enzyme Activation - drug effects</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myometrium - drug effects</subject><subject>Myometrium - enzymology</subject><subject>Myometrium - physiology</subject><subject>Obstetric Labor, Premature - prevention & control</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original Article</subject><subject>Pregnancy</subject><subject>Relaxin - pharmacology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Uterine Contraction - drug effects</subject><subject>Young Adult</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAQhoMo7rr6A7xIwYOn6kySNu1xEb9gwYt6DWmSapd-rEm7uP_elC4sCEIgGfLMO8NDyCXCLQKIOw_AgccAeYzA0zg7InPkjMYgEI_JHPLxDamYkTPv1wBIsyw9JTPMGeZJgnPysYw2nfdVUdtINUW1VbVt-8h1oS47Fzlbq5-qjcL5GhrVRs2ua2zvKlVHqjWRsboyQyi0rWs_Ytuqd905OSlV7e3F_l6Q98eHt_vnePX69HK_XMWaCexjkegs7AIpFKxIlBKCM1OyXGlgqHJkRhtNgbMyN7pAqxRSXpTMWKHykgq2IDdT7sZ134P1vWwqP66iWtsNXgrGEZjgGMjrP-S6G1wblpOUpphlkFEeKJwo7YIVZ0u5cVWj3E4iyNG5nJzL4FyOzmUWeq72yUPRWHPo2EsOAJ0AH77aT-sOo_9P_QX6EYvD</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Baston-Büst, Dunja M.</creator><creator>Hess, Alexandra P.</creator><creator>Hirchenhain, Jens</creator><creator>Krücken, Jürgen</creator><creator>Wunderlich, Frank</creator><creator>Krüssel, Jan S.</creator><creator>Friebe-Hoffmann, Ulrike K.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro</title><author>Baston-Büst, Dunja M. ; Hess, Alexandra P. ; Hirchenhain, Jens ; Krücken, Jürgen ; Wunderlich, Frank ; Krüssel, Jan S. ; Friebe-Hoffmann, Ulrike K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-75c8193060b3b5aa7743df39ac031a913dcdc2043f9dcb1eaa124bf3de7a9f273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Blotting, Western</topic><topic>Cyclooxygenase 1 - biosynthesis</topic><topic>Cyclooxygenase 1 - genetics</topic><topic>Cyclooxygenase 1 - metabolism</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Decidua - cytology</topic><topic>Decidua - drug effects</topic><topic>Decidua - enzymology</topic><topic>Decidua - physiology</topic><topic>Dinoprostone - metabolism</topic><topic>Endocrinology</topic><topic>Enzyme Activation - drug effects</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Myometrium - drug effects</topic><topic>Myometrium - enzymology</topic><topic>Myometrium - physiology</topic><topic>Obstetric Labor, Premature - prevention & control</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original Article</topic><topic>Pregnancy</topic><topic>Relaxin - pharmacology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Uterine Contraction - drug effects</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baston-Büst, Dunja M.</creatorcontrib><creatorcontrib>Hess, Alexandra P.</creatorcontrib><creatorcontrib>Hirchenhain, Jens</creatorcontrib><creatorcontrib>Krücken, Jürgen</creatorcontrib><creatorcontrib>Wunderlich, Frank</creatorcontrib><creatorcontrib>Krüssel, Jan S.</creatorcontrib><creatorcontrib>Friebe-Hoffmann, Ulrike K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baston-Büst, Dunja M.</au><au>Hess, Alexandra P.</au><au>Hirchenhain, Jens</au><au>Krücken, Jürgen</au><au>Wunderlich, Frank</au><au>Krüssel, Jan S.</au><au>Friebe-Hoffmann, Ulrike K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>280</volume><issue>6</issue><spage>961</spage><epage>969</epage><pages>961-969</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE
2
in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.
Methods
In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.
Results
We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.
Conclusions
These effects might potentially contribute to birth-associated induction of contractions in vivo.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19319551</pmid><doi>10.1007/s00404-009-1046-8</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0067 |
| ispartof | Archives of gynecology and obstetrics, 2009-12, Vol.280 (6), p.961-969 |
| issn | 0932-0067 1432-0711 |
| language | eng |
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| subjects | Adult Blotting, Western Cyclooxygenase 1 - biosynthesis Cyclooxygenase 1 - genetics Cyclooxygenase 1 - metabolism Cyclooxygenase 2 - biosynthesis Cyclooxygenase 2 - genetics Cyclooxygenase 2 - metabolism Decidua - cytology Decidua - drug effects Decidua - enzymology Decidua - physiology Dinoprostone - metabolism Endocrinology Enzyme Activation - drug effects Enzyme-Linked Immunosorbent Assay Female Gynecology Human Genetics Humans Immunohistochemistry Medicine Medicine & Public Health Myometrium - drug effects Myometrium - enzymology Myometrium - physiology Obstetric Labor, Premature - prevention & control Obstetrics/Perinatology/Midwifery Original Article Pregnancy Relaxin - pharmacology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Uterine Contraction - drug effects Young Adult |
| title | A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro |
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