Association of the A1936G (rs203462) of A-Kinase Anchoring Protein 10 Polymorphisms With QT Interval Prolongation During Kidney Transplantation

• Published in: Transplantation proceedings 2009-10, Vol.41 (8), p.3036-3038
• Main Authors: Żukowski, M, Bohatyrewicz, R, Biernawska, J, Kotfis, K, Knap, R, Kaczmarczyk, M, Ciechanowicz, A, Binczak, A, Brykczyński, M
• Format: Article
• Language: English
• Subjects: A Kinase Anchor Proteins - genetics; Analysis of Variance; Arrhythmias, Cardiac - epidemiology; Arrhythmias, Cardiac - genetics; Biological and medical sciences; Cardiac dysrhythmias; Cardiology. Vascular system; Electrocardiography; Electrocardiography, Ambulatory - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genetic Variation; Genotype; Heart; Humans; Hydrogen-Ion Concentration; Kidney Transplantation - physiology; Long QT Syndrome - genetics; Medical sciences; Monitoring, Physiologic - methods; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; Tissue, organ and graft immunology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2009.09.016

Abstract Introduction The purpose of this study was to investigate whether the polymorphism in the kinase-binding domain of A-kinase anchoring protein 10 (AKAP10) was related to the risk of occurrence of potentially dangerous arrhythmias during kidney transplant. Methods We performed this prospective observational study with additional patient monitoring during the kidney transplant procedure and in the postoperative period with continuous electrocardiogram (ECG) - (digital holter; ECG monitor type 300-7 Suprima system; Oxford, UK). After manual trace analysis, we performed classification of arrhythmias by interval measurement (including QT correction according to Bazett's formula: Qtc = QT/RR1/2), ST segment analysis within all channels, and analysis of heart rate variability (HRV) parameters (time analysis: SDNN as total rate variability measure, SDANN as long-term variability measure, SDNNindex, rMSSD and pNN50 as short-term variability measure) as well as frequency measure of power width parameters in the spectrum between 0.0033 Hz and 0.4 Hz. Subsequently applying polymerase chain reaction restriction fragment length polymorphism methods, we investigated A1936G (rs203462) AKAP10 polymorphism among 54 kidney recipients. Results Analysis of variance showed that prolongation of the QTc interval associated with the variant genotypes (GG + AG) was significantly greater compared with the AA genotype among kidney recipients ( P = .04). We did not observe a relationship between the AKAP10 polymorphism and other arrhythmias, or clinical or environmental factors. Conclusions Our data suggested that the AKAP10 (rs203462) GG + AG variation was associated with an increased risk of severe arrhythmias during kidney transplantation.