405 nm Absorption Detection in Nanoliter Volumes

Analytical UV absorption detection for microfluidic devices, capillary electrophoresis, and even high-performance liquid chromatography is hampered by the small detection volumes, short absorption paths, and the need to sample at a high rate with a stable background and low noise. Fiber-loop ring-do...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2009-11, Vol.81 (21), p.9048-9054
Main Authors: Waechter, Helen, Bescherer, Klaus, Dürr, Christoph J, Oleschuk, Richard D, Loock, Hans-Peter
Format: Article
Language:English
Subjects:
Absorption
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Exact sciences and technology
Lasers
Myoglobin - analysis
Nanotechnology - methods
Other chromatographic methods
Polystyrene
Spectrometric and optical methods
Spectrophotometry, Ultraviolet - methods
Spectrum analysis
Tartrazine - analysis
Ultraviolet radiation
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Summary:Analytical UV absorption detection for microfluidic devices, capillary electrophoresis, and even high-performance liquid chromatography is hampered by the small detection volumes, short absorption paths, and the need to sample at a high rate with a stable background and low noise. Fiber-loop ring-down spectroscopy (FLRDS) permits absorption detection of dilute liquid samples in volumes as small as a few nanoliters, while being insensitive to light source fluctuations and permitting a millisecond temporal resolution. We demonstrate a FLRDS based detection scheme that is compatible in dimensions (
ISSN:0003-2700
1520-6882
DOI:10.1021/ac901696q