Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

• Published in: BMB reports 2009-10, Vol.42 (10), p.691-696
• Main Authors: Shen, Yan Nan, Kyung Hee University, Seoul, Republic of Korea, Kim, Y.M., Kyung Hee University, Seoul, Republic of Korea, Yun, C.H., Seoul National University, Seoul, Republic of Korea, Moon, Y.S., Jinju National University, Jinju, Republic of Korea, Kim, S.H., Kyung Hee University, Seoul, Republic of Korea
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocyte; Adipocytes - cytology; Adipocytes - metabolism; Animals; Base Sequence; Cell Differentiation; CELLS; CELLULE; CELULAS; E2F1; E2F1 Transcription Factor - genetics; E2F1 Transcription Factor - metabolism; Gene Knockdown Techniques; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Mice; Pref-1; Promoter; Promoter Regions, Genetic - genetics; Protein Binding; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcriptional activation; Transcriptional Activation - genetics
• ISSN: 1976-6696
• DOI: 10.5483/BMBRep.2009.42.10.691

The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in pre-adipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.