Early preconditioning protection against stunning in conscious sheep. Role of KATP channels

The aim of this study was to assess early preconditioning protection against stunning in conscious sheep and analyze the role of ATP-dependent potassium (KATP) channels in the protective mechanism. Chronically instrumented animals were submitted to a 12 min reversible ischemia and 2 h reperfusion. E...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2009-11, Vol.331 (1-2), p.247-257
Main Authors: Lascano, Elena Catalina, Negroni, Jorge A, del Valle, Héctor F
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Adenosine triphosphatase
Animals
Biochemistry
Biomedical and Life Sciences
Cardiology
Hemodynamics
Ischemic Preconditioning, Myocardial
KATP Channels - metabolism
Life Sciences
Male
Medical Biochemistry
Mitochondrial DNA
Myocardial Reperfusion Injury - physiopathology
Myocardial Stunning - metabolism
Myocardial Stunning - physiopathology
Myocardial Stunning - prevention & control
Oncology
Physiology
Potassium
Recovery of Function
Sheep
Time Factors
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Summary:The aim of this study was to assess early preconditioning protection against stunning in conscious sheep and analyze the role of ATP-dependent potassium (KATP) channels in the protective mechanism. Chronically instrumented animals were submitted to a 12 min reversible ischemia and 2 h reperfusion. Early preconditioning, consisting of six 5 min occlusion-5 min reperfusion periods, followed by 45 min normoperfusion before the prolonged ischemia protected against stunning (P < 0.01). In these experimental conditions, current agents used to analyze sarcolemmal (sKATP) and mitochondrial (mKATP) KATP channels could not clearly establish their participation in the protective mechanism. At doses that inhibit sKATP channels they were unable to block preconditioning protection against stunning (glibenclamide) or conversely, blocked preconditioning at doses that do not inhibit these channels (HMR1098). Moreover, both mKATP channel agonists (diazoxide) and antagonists (5HD) protected against stunning, a response that could be due to their effect via an alternative mitochondrial pathway.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-009-0166-6