Hepatitis B virus genotype in Iranian patients with hepatocellular carcinoma

Summary Objective Chronic hepatitis B virus (HBV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC). HBV appears to be the most common cause of HCC in Iran. To date, no study has been carried out on the HBV genotype in Iranian HCC patients. This study was underta...

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Bibliographic Details
Published in:International journal of infectious diseases 2009-11, Vol.13 (6), p.685-689
Main Authors: Aghakhani, Arezoo, Hamkar, Rasool, Zamani, Nastaran, Eslamifar, Ali, Banifazl, Mohammad, Saadat, Alireza, Sofian, Masoomeh, Adibi, Ladan, Irani, Nemat, Mehryar, Matin, Akbari, Hassan Mohammad Hosseini, Purkhayat, Seyed Mohammad Javad, Ramezani, Amitis
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Carcinoma, Hepatocellular - epidemiology
Carcinoma, Hepatocellular - virology
DNA, Viral - analysis
DNA, Viral - isolation & purification
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genotype
Hepatitis B - epidemiology
Hepatitis B - virology
Hepatitis B virus (HBV)
Hepatitis B virus - classification
Hepatitis B virus - genetics
Hepatocellular carcinoma (HCC)
Human viral diseases
Humans
Infectious Disease
Infectious diseases
Iran - epidemiology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Middle Aged
Phylogeny
Polymerase Chain Reaction - methods
Pulmonary/Respiratory
Sequence Analysis, DNA
Tumors
Viral diseases
Viral hepatitis
Young Adult
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Summary:Summary Objective Chronic hepatitis B virus (HBV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC). HBV appears to be the most common cause of HCC in Iran. To date, no study has been carried out on the HBV genotype in Iranian HCC patients. This study was undertaken to determine the HBV genotype in Iranian patients with HCC. Methods Paraffin-embedded tissue samples from 40 patients (31 males and nine females) with HBV-associated HCC were collected from different pathology centers during 2000–2007. Genotyping of HBV was performed by nested PCR-mediated amplification of the target sequence. PCR products were sequenced, and the genotype of each HBV sequence was determined by comparison with sequences of known genotypes in the GenBank. A phylogenetic tree was constructed. Results Phylogenetic analysis revealed that all of the HBV isolates were clustered in genotype D. Conclusions Our results concur with other reports from Iran, all showing that genotype D is the only detectable genotype in the different clinical forms of HBV infection in this country.
ISSN:1201-9712
1878-3511
DOI:10.1016/j.ijid.2008.10.009