Enzymatic AND-gate based on electrode-immobilized glucose-6-phosphate dehydrogenase: Towards digital biosensors and biochemical logic systems with low noise

Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for “digital” (threshold) sensing/actuati...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-12, Vol.25 (4), p.695-701
Main Authors: Privman, Vladimir, Pedrosa, Valber, Melnikov, Dmitriy, Pita, Marcos, Simonian, Aleksandr, Katz, Evgeny
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensor network
Biosensors
Biotechnology
Computers, Molecular
Digital sensor
Enzyme logic
Enzymes, Immobilized - chemistry
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Glucosephosphate Dehydrogenase - chemistry
Logic gate
Methods. Procedures. Technologies
Microelectrodes
Signal Processing, Computer-Assisted - instrumentation
Various methods and equipments
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Summary:Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for “digital” (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.08.014