Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate

A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 μg/ml inhibited adipogenesis whereas fully differe...

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Bibliographic Details
Published in:Biotechnology letters 2009-12, Vol.31 (12), p.1837-1841
Main Authors: Shilpa, Kusampudi, Sangeetha, Kadapakkam Nandabalan, Muthusamy, Velusamy Shanmuganathan, Sujatha, Sundaresan, Lakshmi, Baddireddi Subadhra
Format: Article
Language:English
Subjects:
Adipocytes - drug effects
Adipogenesis - drug effects
Animals
Applied Microbiology
Biochemistry
Biological and medical sciences
Biological products
Biomedical and Life Sciences
Biotechnology
Cell Line
Costus
Costus - chemistry
Diabetes
Fatty Acids - isolation & purification
Fatty Acids - pharmacology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling
Gene Expression Regulation - drug effects
Hypoglycemic Agents - isolation & purification
Hypoglycemic Agents - pharmacology
Insulin
Life Sciences
Mice
Microbiology
Original Research Paper
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Signal Transduction - drug effects
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Summary:A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 μg/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-009-0105-3