Improvement of direct agglutination test (DAT) for laboratory diagnosis of visceral leishmaniasis in Brazil

We previously standardized the direct agglutination test (DAT) to detect anti- Leishmania chagasi promastigote antibodies (DAT-LPC) with good sensitivity and specificity for diagnosing visceral leishmaniasis (VL). In this paper, we present a technical upgrade by introducing some modifications into t...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 2009-12, Vol.103 (12), p.1279-1281
Main Authors: Oliveira, Edward, Pedras, Mariana Junqueira, Assis, Isabella Elias Morais de, Rabello, Ana
Format: Article
Language:English
Subjects:
Agglutination Tests - standards
Antibodies, Protozoan - blood
Antigens, Protozoan - blood
Biological and medical sciences
Brazil
DAT
Diagnosis
Female
General aspects
Human protozoal diseases
Humans
Infectious diseases
Leishmania donovani - isolation & purification
Leishmaniasis, Visceral - diagnosis
Leshmaniasis
Male
Medical sciences
Parasitic diseases
Protozoal diseases
Reproducibility of Results
Sensitivity and Specificity
Serologic test
Visceral leishmaniasis
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Summary:We previously standardized the direct agglutination test (DAT) to detect anti- Leishmania chagasi promastigote antibodies (DAT-LPC) with good sensitivity and specificity for diagnosing visceral leishmaniasis (VL). In this paper, we present a technical upgrade by introducing some modifications into the antigen preparation. This antigen was evaluated in DAT (DAT-Mod) using 61 sera samples from VL patients and 96 samples from patients with other diseases. The DAT-Mod presented a cut-off of 1:100, satisfactory reproducibility (VC
ISSN:0035-9203
1878-3503
DOI:10.1016/j.trstmh.2009.04.007