Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis (2-DE)

Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3...

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Published in:Polish journal of microbiology 2009, Vol.58 (3), p.205-209
Main Authors: Bugla-Płoskońska, Gabriela, Futoma-Kołoch, Bozena, Skwara, Aleksandra, Doroszkiewicz, Włodzimierz
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - chemistry
Detergents - chemistry
E coli
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Escherichia coli - metabolism
Membrane proteins
Outer membrane proteins
Zwitterions
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container_issue 3
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container_title Polish journal of microbiology
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creator Bugla-Płoskońska, Gabriela
Futoma-Kołoch, Bozena
Skwara, Aleksandra
Doroszkiewicz, Włodzimierz
description Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).
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2544-4646
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subjects Bacterial Outer Membrane Proteins - chemistry
Detergents - chemistry
E coli
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Escherichia coli - metabolism
Membrane proteins
Outer membrane proteins
Zwitterions
title Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis (2-DE)
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