Adenosine Triphosphoric Acid as a Factor of Nervous Regulation of Na⁺/K⁺/2Cl⁻ Cotransport in Rat Skeletal Muscle Fibers

Exogenous adenosine triphosphoric acid produces a biphasic effect on the resting membrane potential of muscle fibers in rat diaphragm. Depolarization of the sarcolemma observed 10 min after application of adenosine triphosphoric acid results from activation of Na⁺/K⁺/2Cl⁻ cotransport. The increase i...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2009-05, Vol.147 (5), p.583-586
Main Authors: Naumenko, N. V, Uzinskaya, K. V, Shakirzyanova, A. V, Urazaev, A. Kh, Zefirov, A. L
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
Adenosine Triphosphate - pharmacology
adenosine triphosphoric acid
Animals
Biological Transport - drug effects
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Cell Biology
chloride cotransport
Chlorides - metabolism
Internal Medicine
Laboratory Medicine
Male
membrane potential
Membrane Potentials - drug effects
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
neuromuscular synapse
P2Y receptors
Pathology
Potassium - metabolism
Protein Kinase C - metabolism
Rats
Receptors, Purinergic P2 - metabolism
Sarcolemma - drug effects
Sarcolemma - metabolism
Sodium - metabolism
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Summary:Exogenous adenosine triphosphoric acid produces a biphasic effect on the resting membrane potential of muscle fibers in rat diaphragm. Depolarization of the sarcolemma observed 10 min after application of adenosine triphosphoric acid results from activation of Na⁺/K⁺/2Cl⁻ cotransport. The increase in chloride cotransport is related to activation of postsynaptic P2Y receptors and protein kinase C. Repolarization of the membrane develops 40 min after treatment with adenosine triphosphoric acid and after 50 min the resting membrane potential almost returns the control level. This increase in the resting membrane potential of the sarcolemma is probably associated with activation of the Na⁺/K⁺ pump and increase in membrane permeability for chlorine ions in response to longterm activity of Cl⁻ cotransport. Thus, adenosine triphosphoric acid co-secreted with acetylcholine in the neuromuscular synapse probably plays a role in the regulation resting membrane potential and cell volume of muscle fibers.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0575-2