Administration of peripheral blood mononuclear cells into the uterine horn to improve pregnancy rate following bovine embryo transfer

Embryo transfer (ET) has been used to improve reproductive efficiency and genetic make-up in bovine species. However, the success rate of ET has not been improved since its inception. Here we examined whether administration of autologous peripheral blood mononuclear cells (PBMCs) into the uterine ho...

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Bibliographic Details
Published in:Animal reproduction science 2010, Vol.117 (1), p.18-23
Main Authors: Ideta, Atsushi, Sakai, Shin-ichi, Nakamura, Yuuki, Urakawa, Manami, Hayama, Koh, Tsuchiya, Kanami, Fujiwara, Hiroshi, Aoyagi, Yoshito
Format: Article
Language:English
Subjects:
animal health
animal performance
Animals
blood cells
Bovine
Cattle - physiology
conception
cows
Embryo transfer
Embryo Transfer - methods
Embryo Transfer - veterinary
Estrous Cycle
Female
female fertility
immunity
immunostimulants
interleukin-1
Interleukin-1alpha - analysis
Interleukin-1beta - analysis
interleukin-8
Interleukin-8 - analysis
Leukocytes, Mononuclear - chemistry
Leukocytes, Mononuclear - transplantation
Peripheral blood mononuclear cells
Pregnancy
Pregnancy Rate
RNA, Messenger - analysis
therapeutics
transcriptional activation
Uterus
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Summary:Embryo transfer (ET) has been used to improve reproductive efficiency and genetic make-up in bovine species. However, the success rate of ET has not been improved since its inception. Here we examined whether administration of autologous peripheral blood mononuclear cells (PBMCs) into the uterine horn can improve pregnancy rates following bovine ET. First we determined that the abundance of interleukin (IL)-1α, IL-1β and IL-8 transcripts in PBMCs was greatest after 24 h of culture. PBMCs that had been cultured for 24 h were gently administered non-surgically to the uterine horn ipsilateral to the corpus luteum on day 4 of the estrous cycle. On day 7, the ET was carried out and the pregnancy rate in the PBMC-treated group was compared with that in the non-treated group. The pregnancy rate on day 60 in the PBMC-treated group (76.7%, 56/73) was significantly higher than that in the non-treated group (59.7%, 43/72, p < 0.05). These results indicate that administration of autologous PBMCs into the uterine horn improves pregnancy rates following bovine ET.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2009.04.004