In vitro Adipogenic Differentiation of Preadipocytes Varies with Differentiation Stimulus, Culture Dimensionality, and Scaffold Composition

Despite the rapidly growing body of work on stem cell–based adipose tissue engineering, there remains much to be learned about the role of the scaffold and culture environments in directing the adipogenic differentiation of cells. The present study examined how various culture environments and diffe...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part A 2009-11, Vol.15 (11), p.3389-3399
Main Authors: Stacey, D. Heath, Hanson, Summer E., Lahvis, Garet, Gutowski, Karol A., Masters, Kristyn S.
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - physiology
Adipogenesis - physiology
Biomarkers
Cell culture
Cell Culture Techniques - methods
Cell Differentiation
Cells, Cultured
Comparative studies
Extracellular Matrix - physiology
Humans
Original Articles
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Tissue engineering
Tissue Engineering - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite the rapidly growing body of work on stem cell–based adipose tissue engineering, there remains much to be learned about the role of the scaffold and culture environments in directing the adipogenic differentiation of cells. The present study examined how various culture environments and differentiation stimuli (traditional differentiation medium [DM] and coculture with mature adipocytes) impacted the adipogenic differentiation of human preadipocytes, with studies progressing from two-dimensions (2D) to three-dimensions (3D) in vitro . Assays for adipogenic markers (leptin, adiponectin, and glycerol) and Oil Red O staining were used to assess differentiation. After 16 days of 2D culture, adipogenesis was substantially greater when preadipocytes were cocultured with adipocytes rather than treated with DM. In a 3D in vitro environment, the production of adipogenic markers was significantly elevated relative to 2D conditions, and the coculture condition continued to stimulate greater adipogenesis. Alterations in 3D scaffold physical properties had only a minimal effect on the function of mature adipocytes, but significantly impacted the ability of preadipocytes to undergo adipogenic differentiation in vitro . These alterations in scaffold environment and in medium conditions, particularly the application of adipocyte/preadipocyte coculture methods in lieu of traditional DM, may provide further means for optimizing adipogenic outcomes in vitro and in vivo .
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2008.0293