FoxD5 mediates anterior–posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis

The transcription factor FoxD5 is expressed in the paraxial mesoderm of zebrafish. However, the roles of FoxD5 in anterior pre-somitic mesoderm (PSM) during somitogenesis are unknown. We knocked down FoxD5 in embryos, which resulted in defects of the newly formed somites, including loss of the strip...

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Bibliographic Details
Published in:Developmental biology 2009-12, Vol.336 (2), p.232-245
Main Authors: Lee, Hung-Chieh, Tseng, Wei-An, Lo, Fang-Yi, Liu, Tzu-Ming, Tsai, Huai-Jen
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Body Patterning
Danio rerio
DNA Primers
Fgf
Fibroblast Growth Factors - metabolism
Forkhead Transcription Factors - genetics
Forkhead Transcription Factors - physiology
FoxD5
Freshwater
Gene Knockdown Techniques
Immunohistochemistry
Polymerase Chain Reaction
RNA, Messenger - genetics
Signal Transduction
Somites - metabolism
Somitogenesis
Zebrafish
Zebrafish - embryology
Zebrafish Proteins - genetics
Zebrafish Proteins - physiology
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Summary:The transcription factor FoxD5 is expressed in the paraxial mesoderm of zebrafish. However, the roles of FoxD5 in anterior pre-somitic mesoderm (PSM) during somitogenesis are unknown. We knocked down FoxD5 in embryos, which resulted in defects of the newly formed somites, including loss of the striped patterns of anterior–posterior polarity genes deltaC, notch2, notch3 and EphB2a, as well as the absence of mespa expression in S-I. Also, the expression of mespb exhibited a ‘salt and pepper’ pattern, indicating that FoxD5 is necessary for somite patterning in anterior PSM. Embryos were treated with SU5402, an Fgf receptor (FGFR) inhibitor, resulting in reduction of FoxD5 expression. This finding was consistent with results obtained from Tg(hsp70l:dnfgfr1-EGFP)pd1 embryos, whose dominant-negative form of FGFR1 was produced by heat-induction. Loss of FoxD5 expression was observed in the embryos injected with fgf3-/fgf8-double-morpholinos (MOs). Excessive FoxD5 mRNA could rescue the defective expression levels of mespa and mespb in fgf3-/fgf8-double morphants, suggesting that Fgf signaling acts as an upstream modulator of FoxD5 during somitogenesis. We concluded that FoxD5 is required for maintaining anterior–posterior polarity within a somite and that the striped pattern of FoxD5 in anterior PSM is mainly regulated by Fgf. An Fgf-FoxD5-Mesps signaling network is therefore proposed.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2009.10.001