Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap-3) by a new class-II piggyBac-related insect transposon

A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni...

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Bibliographic Details
Published in:Insect molecular biology 2009-12, Vol.18 (6), p.747-757
Main Authors: Carpes, M.P, Nunes, J.F, Sampaio, T.L, Castro, M.E.B, Zanotto, P.M.A, Ribeiro, B.M
Format: Article
Language:English
Subjects:
AgMNPV
Amino Acid Sequence
Animals
Anticarsia gemmatalis
Base Sequence
DNA Primers - genetics
DNA Transposable Elements - genetics
DNA Transposable Elements - physiology
Electrophoresis, Agar Gel
Inhibitor of Apoptosis Proteins - metabolism
Molecular Sequence Data
Moths - virology
Mutation - genetics
Nucleopolyhedrovirus - genetics
Phylogeny
piggyBac
Sequence Alignment
Sequence Analysis, DNA
Species Specificity
transposon
vApAg
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Summary:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2009.00917.x