Pax-3 and Pax-7 Label Muscle Progenitor Cells During Myotomal Myogenesis in Coregonus lavaretus (Teleostei: Coregonidae)

• Published in: Anatomia, histologia, embryologia histologia, embryologia, 2009-12, Vol.38 (6), p.411-418
• Main Authors: Kacperczyk, A, Jagla, T, Daczewska, M
• Format: Article
• Language: English
• Subjects: Animals; Mesoderm - embryology; Muscle Development - physiology; Muscle Fibers, Fast-Twitch - physiology; Muscle Fibers, Skeletal; Muscle, Skeletal - embryology; Muscle, Skeletal - growth & development; Myoblasts - metabolism; MyoD Protein - metabolism; Myosin Heavy Chains - metabolism; Notochord - embryology; Paired Box Transcription Factors - genetics; Paired Box Transcription Factors - metabolism; PAX3 Transcription Factor; PAX7 Transcription Factor - genetics; PAX7 Transcription Factor - metabolism; Salmonidae - embryology; Salmonidae - metabolism; Satellite Cells, Skeletal Muscle - physiology; Somites - embryology; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism
• ISSN: 0340-2096; 1439-0264
• DOI: 10.1111/j.1439-0264.2009.00961.x

In Coregonus lavaretus, prior the mesoderm segmentation, in cells adjacent to the notochord called adaxial cells MyoD and slow myosin heavy chain (MyHC-slow) proteins were observed. After somite formation, adaxial cells migrate towards the lateral part of the myotome and form a layer of red muscles. Deeper cells differentiate into white muscle fibres. In situ hybridization using Pax-3 molecular probe revealed, that after somitogenesis, Pax-3 is expressed in a layer of cells superficial to the myotome resembling the "external cells" (found in many teleosts species) or dermomyotome described in Amniota. During later developmental stages Pax-3 gene is expressed in cells in intermyotomal space and then in myoblasts between myotubes. In these cells Pax-7 protein was also observed. Pax-3/7 positive cells which have migrated into the myotomes differentiate into satellite cells/secondary myoblasts and participate in hypertrophic and hyperplastic growth of muscles.