Vasopressin-dependent short-term regulation of aquaporin 4 expressed in Xenopus oocytes

Abstract Aquaporin 4 (AQP4) is abundantly expressed in the perivascular glial endfeet in the central nervous system (CNS), where it is involved in the exchange of fluids between blood and brain. At this location, AQP4 contributes to the formation and/or the absorption of the brain edema that may ari...

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Bibliographic Details
Published in:Neuroscience 2009-12, Vol.164 (4), p.1674-1684
Main Authors: Moeller, H.B, Fenton, R.A, Zeuthen, T, MacAulay, N
Format: Article
Language:English
Subjects:
Animals
Aquaporin 4 - biosynthesis
Aquaporin 4 - genetics
aquaporins
Biological and medical sciences
brain
edema
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Humans
Immunoblotting
Immunohistochemistry
In Vitro Techniques
Membrane Potentials
Neurology
oocytes
Oocytes - physiology
Patch-Clamp Techniques
Permeability
Phosphorylation
Protein Kinase C - metabolism
Rats
Receptors, Vasopressin - biosynthesis
Receptors, Vasopressin - genetics
Serine - metabolism
Signal Transduction
Vasopressins - physiology
Vertebrates: nervous system and sense organs
Water - metabolism
water homeostasis
Xenopus laevis
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Summary:Abstract Aquaporin 4 (AQP4) is abundantly expressed in the perivascular glial endfeet in the central nervous system (CNS), where it is involved in the exchange of fluids between blood and brain. At this location, AQP4 contributes to the formation and/or the absorption of the brain edema that may arise following pathologies such as brain injuries, brain tumours, and cerebral ischemia. As vasopressin and its G-protein-coupled receptor (V1a R) have been shown to affect the outcome of brain edema, we have investigated the regulatory interaction between AQP4 and V1a R by heterologous expression in Xenopus laevis oocytes. The water permeability of AQP4/V1a R-expressing oocytes was reduced in a vasopressin-dependent manner, as a result of V1a R-dependent internalization of AQP4. Vasopressin-dependent internalization was not observed in AQP9/V1a R-expressing oocytes. The regulatory interaction between AQP4 and V1a R involves protein kinase C (PKC) activation and is reduced upon mutation of Ser180 on AQP4 to an alanine. Thus, the present study demonstrates at the molecular level a functional link between the vasopressin receptor V1a R and AQP4. This functional interaction between AQP4 and V1a R may prove to be a potential therapeutic target in the prevention and treatment of brain edema.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2009.09.072