Serological and molecular evidence of hepatitis E virus in swine in Brazil

Active hepatitis E virus (HEV) infections in two Brazilian swine herds were investigated. In study 1, 26 piglets born to five anti-HEV positive sows were monitored from birth to post-partum week 22. Serum samples were screened for the detection of anti-HEV antibodies and a nested RT-PCR used to exam...

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Bibliographic Details
Published in:The veterinary journal (1997) 2009-12, Vol.182 (3), p.474-480
Main Authors: dos Santos, Debora Regina Lopes, Vitral, Claudia Lamarca, de Paula, Vanessa Salete, Marchevsky, Renato Sergio, Lopes, Juliana Freitas, Gaspar, Ana Maria Coimbra, Saddi, Telma Michella, Júnior, Nilton Cecílio de Mesquita, Guimarães, Flavio de Rezende, Júnior, João Garcia Caramori, Ximenes, Lia Laura Lewis, Souto, Francisco José Dutra, Pinto, Marcelo Alves
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
antibodies
Antibodies, Viral - blood
Brazil
Brazil - epidemiology
Disease Reservoirs - veterinary
Disease Reservoirs - virology
Female
Gene Amplification
Genotype
Hepatitis E - epidemiology
Hepatitis E - transmission
Hepatitis E - veterinary
Hepatitis E - virology
Hepatitis E virus
Hepatitis E virus - classification
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
histology
Infectious Disease Transmission, Vertical - veterinary
isolation
Male
Molecular Epidemiology
passive immunity
Phylogeny
Pregnancy
Public Health
Retrospective Studies
reverse transcriptase polymerase chain reaction
RNA, Viral - analysis
seroconversion
serodiagnosis
Seroepidemiologic Studies
Swine
swine diseases
Swine Diseases - epidemiology
Swine Diseases - transmission
Swine Diseases - virology
viral hepatitis
Zoonoses
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Summary:Active hepatitis E virus (HEV) infections in two Brazilian swine herds were investigated. In study 1, 26 piglets born to five anti-HEV positive sows were monitored from birth to post-partum week 22. Serum samples were screened for the detection of anti-HEV antibodies and a nested RT-PCR used to examine the HEV genome. Passive transfer of immunity was confirmed. At week 22, 23/26 (88.4%) of the piglets had seroconverted. Genome amplification was achieved in a feces pool from one holding pen and in one serum sample, both from 13-week-old animals. Histology was suggestive of a potential HEV infection. In the second study, 47 piglets born to six anti-HEV-positive sows were monitored after weaning. Seroconversion was determined in eight animals at 6–8 weeks of age. HEV RNA was detected in two pools from a holding pen for 12–16-week-old animals. Brazilian isolates were classified as genotype 3. This is the first molecular evidence of HEV infection in Brazilian pig herds.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2008.08.001