Isotachophoresis of nucleic acids in agarose gel rods

A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters of a solution at so low concentration...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow) 2009-11, Vol.74 (11), p.1285-1288
Main Authors: Kondratova, V. N, Botezatu, I. V, Shelepov, V. P, Lichtenstein, A. V
Format: Article
Language:English
Subjects:
Acids
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Blood Chemical Analysis
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - genetics
Electrophoresis, Agar Gel - methods
Humans
Life Sciences
Microbiology
Nucleic acids
Polymers
Research methodology
Ribonucleic acid
RNA
RNA - chemistry
RNA - genetics
Short Communications
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters of a solution at so low concentration that their isolation by other methods is hardly attainable and fraught with losses are brought together into one visible narrow band when put in a discontinuous electric field. Polynucleotides migrate in dilute (0.1%) semifluid agarose gel that permits easy quantitative isolation of the band of interest. Resulting DNA can be used directly in PCR. The suggested method for isolation of micro amounts of nucleic acids from dilute solutions can be applied to forensic and clinical research and cancer gene diagnostics by the analysis of fragmented circulating DNA from bodily fluids.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297909110169