Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model

Abstract Hypoxic tumour regions often contain viable cells that are more resistant to chemotherapy and/or radiotherapy, making it of key importance to analyse new combination treatments under both normoxic and hypoxic conditions. In this study, the impact of moderate hypoxia and anoxia on cellular c...

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Bibliographic Details
Published in:Cancer letters 2009-12, Vol.286 (2), p.180-188
Main Authors: Wouters, An, Pauwels, Bea, Lambrechts, Hilde A.J, Pattyn, Greet G.O, Ides, Johan, Baay, Marc, Meijnders, Paul, Dewilde, Sylvia, Vermorken, Jan B, Lardon, Filip
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Apoptosis - radiation effects
Blotting, Western
Cell cycle
Cell Cycle - drug effects
Cell Cycle - radiation effects
Cell Hypoxia
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Proliferation - radiation effects
Cell Survival - drug effects
Cell Survival - radiation effects
Hematology, Oncology and Palliative Medicine
Humans
Hypoxia
Medical research
Oxygen - metabolism
Oxygen - pharmacology
p53
Radioresistance
Reverse Transcriptase Polymerase Chain Reaction
Rodents
Studies
Time Factors
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
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Summary:Abstract Hypoxic tumour regions often contain viable cells that are more resistant to chemotherapy and/or radiotherapy, making it of key importance to analyse new combination treatments under both normoxic and hypoxic conditions. In this study, the impact of moderate hypoxia and anoxia on cellular characteristics was investigated in isogenic A549 cells differing in p53 status. VEGF expression, doubling time, cell cycle distribution, induction of apoptosis and p53 protein expression were evaluated. Radiation survival curves yielded an oxygen enhancement ratio of 1.16–1.67. In conclusion, an in vitro hypoxia model that will be highly useful to analyse chemoradiation interactions is presented.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2009.05.026