Completed sequence of plasmid pIP501 and origin of spontaneous deletion derivatives

The sequence of plasmid pIP501 (30,603 bp) was completed using previously published and newly acquired data. The sites at which two spontaneous deletions had occurred were identified. One was between tracts of repeated heptamers and the other between regions of secondary structure associated with pl...

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Bibliographic Details
Published in:Plasmid 2003-07, Vol.50 (1), p.28-35
Main Authors: Thompson, J.K., Collins, M.A.
Format: Article
Language:English
Subjects:
Base Sequence
Conjugative
Enterococcus faecalis - genetics
Genes, Bacterial - genetics
Molecular Sequence Data
Plasmid pIP501
Plasmids - genetics
Sequence Analysis, DNA
Sequence Deletion - genetics
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Summary:The sequence of plasmid pIP501 (30,603 bp) was completed using previously published and newly acquired data. The sites at which two spontaneous deletions had occurred were identified. One was between tracts of repeated heptamers and the other between regions of secondary structure associated with plasmid replication. A high level of identity (>95%) between plasmid pIP501 and part of plasmid pRE25, which had been isolated from Enterococcus faecalis associated with a food source, was confirmed.
ISSN:0147-619X
1095-9890
DOI:10.1016/S0147-619X(03)00042-8