Differential responses to oxidative stress and calcium influx on expression of the transforming growth factor-β family in myoblasts and myotubes

Changes in gene expression of TGF‐β family members and their receptors in response to treatment with H2O2 and a calcium ionophore, A23187, were examined in C2C12 myoblasts and myotubes. The expression of Myf5, an initial regulator of myogenesis, was increased by A23187, and H2O2 inhibited the up‐reg...

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Published in:Cell biochemistry and function 2009-12, Vol.27 (8), p.578-582
Main Authors: Furutani, Yuuma, Murakami, Masaru, Funaba, Masayuki
Format: Article
Language:English
Subjects:
Animals
Ca2+ influx
Calcium - metabolism
Cell Differentiation
Cell Line
Gene Expression Regulation - drug effects
Hydrogen Peroxide - pharmacology
Mice
Multigene Family - drug effects
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
myoblasts
Myoblasts - cytology
Myoblasts - drug effects
Myoblasts - metabolism
myotubes
Oxidative Stress
TGF-beta Superfamily Proteins - genetics
TGF-beta Superfamily Proteins - metabolism
TGF-β family
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Summary:Changes in gene expression of TGF‐β family members and their receptors in response to treatment with H2O2 and a calcium ionophore, A23187, were examined in C2C12 myoblasts and myotubes. The expression of Myf5, an initial regulator of myogenesis, was increased by A23187, and H2O2 inhibited the up‐regulation of Myf5. Treatment with H2O2 decreased the expression of MHC IIb, a protein component of the myofibrils, irrespective of the presence of A23187, suggesting an inhibitory role of oxidative stress for myogenesis. Expression of ligands and receptors for the TGF‐β family was modulated in response to H2O2 and A23187. Treatment with H2O2 decreased expression of TGF‐β3, BMP‐4, ALK4, ALK5, and ActRIIB, and increased expression of inhibin α and inhibin βA in either the myoblast stage or the myotube stage, or both. A23187 potentiated down‐regulation of BMP‐4 and ALK4 expression, and up‐regulation of TGF‐β1, TGF‐β2, inhibin α, inhibin βA, ALK2, and ALK3 expression. These results indicate that oxidative stress and Ca2+ influx affect expression of the TGF‐β family in C2C12 myoblasts and myotubes. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.1614