The effect of high dose of cortisol on glucose-6-phosphatase and fructose-1,6-bisphosphatase activity, and glucose and fructose-2,6-bisphosphate concentration in carp tissues ( Cyprinus carpio L.)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2003-07, Vol.135 (3), p.485-491
• Main Authors: Dziewulska-Szwajkowska, Daria, Łozińska-Gabska, Małgorzata, Adamowicz, Anna, Wojtaszek, Jan, Dzugaj, Andrzej
• Format: Article
• Language: English
• Subjects: Adenosine Monophosphate - metabolism; AMP; Animals; Blood Glucose - metabolism; Carp; Carps - blood; Carps - metabolism; Cortisol; Cyprinus carpio; Dose-Response Relationship, Drug; Freshwater; Fructose-1,6-bisphosphatase; Fructose-2,6-bisphosphate; Fructose-Bisphosphatase - metabolism; Fructosediphosphates - metabolism; Gluconeogenesis; Glucose-6-phosphatase; Glucose-6-Phosphatase - metabolism; Hydrocortisone - administration & dosage; Hydrocortisone - blood; Hydrocortisone - pharmacology; Liver - chemistry; Liver - drug effects; Liver - enzymology; Muscles - chemistry; Muscles - drug effects; Muscles - enzymology
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/S1096-4959(03)00112-X

The effect of a high dose of cortisol (200 mg kg −1 body mass) on juvenile carp was investigated. The activity of glucose-6-phosphatase in liver and of fructose-1,6-bisphosphatase in liver, kidney and muscle, the serum glucose and fructose-2,6-bisphosphate concentration as well as the serum concentration of the injected hormone were measured after 24, 72 and 216 h after intraperitoneal cortisol injection. The activities of fructose-1,6-bisphosphatase in liver and kidney and glucose-6-phosphatase in liver were elevated in comparison with the control, while the fructose-1,6-bisphosphatase activity in the muscle tissue was unchanged. After cortisol injection, the serum glucose level was nearly two times higher after 24 and 72 h and was still 50% higher after 216 h compared with controls. In contrast, the liver fructose-2,6-bisphosphate concentration was unchanged after 24 h. More than two times higher fructose-2,6-bisphosphate concentration was observed in liver after 72 h and it was still elevated after 216 h after the cortisol injection.