Gly482Ser polymorphism of the coactivator-1alpha of the activated receptor of peroxisome gamma proliferation in individuals from Maracaibo, Venezuela

• Published in: Investigación clínica 2009-09, Vol.50 (3), p.285-294
• Main Authors: Zambrano, Mariana, Fernández, Erika, López, Maciel, Rangel, Annell, de Romero, Pilar, Fernández, Virginia, Marina Morales, Luz, Molero-Conejo, Emperatriz, Connell, Lissett, Raleigh, Xiomara, Aranguren-Mendez, José
• Format: Article
• Language: Spanish
• Subjects: Adult; Amino Acid Substitution; Blood Glucose - analysis; Cholesterol - blood; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Female; Genotype; Heat-Shock Proteins - genetics; Humans; Hypertriglyceridemia - blood; Hypertriglyceridemia - etiology; Insulin - blood; Insulin Resistance - genetics; Male; Middle Aged; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Transcription Factors - genetics; Triglycerides - blood; Urban Population - statistics & numerical data; Venezuela - epidemiology
• ISSN: 0535-5133

The aim of this study was to determine the association of the Gly482Ser polymorphism of the PGC-1 gene with insulin resistance and type 2 diabetes mellitus in subjects from the city of Maracaibo. The study was performed on 64 no-diabetic subjects (36 without insulin resistance and 28 with insulin resistance) and 13 with type 2 diabetes. A clinical and nutritional history was carried out and the evaluation of anthropometric parameters was included. Fasting serum glucose, fasting serum insulin, total cholesterol, HDL-C and LDL-C, were measured. The Gly482Ser polymorphism was detected by PCR-RFLP. It was found that the allelic frequencies for A and G were 0.36 and 0.64, respectively. The population was found in genetic equilibrium of Hardy Weinberg. The genotypes of the polymorphism Gly482Ser were not associated with insulin resistance and type 2 diabetes mellitus (OR = 1.320, p = 0.74; OR = 2, p = 0.47 respectively). Nevertheless, the diabetic subjects with the genotype AA presented values of LDL-C higher (p < 0.05) than the individuals with the genotypes GG and GA. The diabetics with the genotype GA showed significantly higher concentrations of triglycerides (>150 mg/dL) compared with the genotype GG. According to the results, the polymorphism Gly482Ser of the PGC-1 gene would be able to contribute to the cardiovascular risk in type 2 diabetics, while in the insulin resistant individuals, this polymorphism was not associated with cardiovascular risk factors.