Fluorimetric lead detection in a microfluidic device

A microfabricated device has been developed for the selective detection of lead in water. It is based on the use of a selective and sensitive fluorescent molecular sensor for lead (Calix-DANS4) which contains a calix[4]arene bearing four dansyl groups. The microchip-based lead sensor contains a Y-sh...

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Bibliographic Details
Published in:Lab on a chip 2009-01, Vol.9 (19), p.2818-2823
Main Authors: Zhao, Liyun, Wu, Ting, Lefèvre, Jean-Pierre, Leray, Isabelle, Delaire, Jacques A
Format: Article
Language:English
Subjects:
Equipment Design
Fluorescent Dyes - chemistry
Fluorometry - instrumentation
Fluorometry - methods
Lead - analysis
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Water - chemistry
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Summary:A microfabricated device has been developed for the selective detection of lead in water. It is based on the use of a selective and sensitive fluorescent molecular sensor for lead (Calix-DANS4) which contains a calix[4]arene bearing four dansyl groups. The microchip-based lead sensor contains a Y-shape microchannel equipped with a passive mixer and moulded on a glass substrate. An optimization of the microcircuit length has been performed in order to have a full complexation of the Calix-DANS4. The detection is performed by using a configuration in which the sensing molecules are excited by two optical fibres, each one connected to a 365 nm UV LED, and the light collection is made by another optical fibre with a photomultiplier. By using this configuration we have shown the possibility to detect lead with a detection limit of 5 ppb. The effect of interfering cations such as calcium has been evaluated. The obtained measurements have been validated by an alternative method (ASV).
ISSN:1473-0197
1473-0189
DOI:10.1039/b904641k