The Chlamydomonas Chloroplast HLP Protein Is Required for Nucleoid Organization and Genome Maintenance

• Published in: Molecular plant 2009-11, Vol.2 (6), p.1223-1232
• Main Authors: Karcher, Daniel, Köster, Dietrich, Schadach, Anne, Klevesath, Anja, Bock, Ralph
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Cell Nucleus - genetics; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - genetics; Chloroplast; Chloroplasts - genetics; Chloroplasts - physiology; Cloning, Molecular; Cyanobacteria - genetics; DNA-binding protein; DNA结合蛋白; genome maintenance; Genome, Plant; HLP; Molecular Sequence Data; nucleoid; Plant Proteins - genetics; Plant Proteins - physiology; Restriction Mapping; RNA干扰; Sequence Alignment; Sequence Homology, Amino Acid; 叶绿体基因组; 基因拷贝数; 核基因组; 核蛋白; 组织; 维护
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/ssp083

The chloroplasts genome (plastome) occurs at high copy numbers per cell. Several chloroplast genome copies are densely packed into nucleoprotein particles called nucleoids. How genome packaging occurs and which proteins organize chloroplast nucleoids are largely unknown. Here, we have analyzed the Chlamydomonas reinhardtii homolog of the bacterial architectural DNA-binding protein HU, the histone-like protein HLP. We show that the Chlamydomonas HLP protein is targeted to chloroplasts and associates with nucleoids. Knockdown of HLP gene expression by RNA interference (RNAi) alters the structure of chloroplast nucleoids and appears to reduce the level of compaction of chloroplast DNA. Unexpectedly, also chloroplast genome copy numbers are significantly decreased in the RNAi strains, suggesting that, in addition to its architectural role in nucleoid formation, the HLP protein is also involved in chloroplast genome maintenance.