Growth, death, and oxygen uptake kinetics of Pichia stipitis on xylose

Pichia stipitis NRRL Y-7124 has potential application in the fermentation of xylose-rich waste streams produced by wood hydrolysis. Kinetic models of cell growth, death, and oxygen uptake were investigated in batch and oxygenlimited continuous cultures fed a rich synthetic medium. Variables included...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1991-04, Vol.37 (10), p.973-980
Main Authors: Slininger, P.J. (Northern Regional Research Center, USDA, ARS, Peoria, IL), Branstrator, L.E, Bothast, R.J, Okos, M.R, Ladisch, M.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
CELLULE
CELULAS
CRECIMIENTO
CROISSANCE
dissolved oxygen
ETANOL
ETHANOL
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
OXIGENO
oxygen
OXYGENE
PICHIA
PRODUCCION
PRODUCTION
XILOSA
XYLOSE
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Summary:Pichia stipitis NRRL Y-7124 has potential application in the fermentation of xylose-rich waste streams produced by wood hydrolysis. Kinetic models of cell growth, death, and oxygen uptake were investigated in batch and oxygenlimited continuous cultures fed a rich synthetic medium. Variables included rates of dilution (D) and oxygen transfer (K,a) and concentrations of xylose (X), ethanol (E), and dissolved oxygen (Cox). Sustained cell growth required the presence of oxygen. Given excess xylose, specific growth rate (micro) was a Monod function of Cox. Specific oxygen uptake rate was proportional to micro by a yield coefficient relating biomass production to oxygen consumption; but oxygen uptake for maintenance was negligible. Thus steady-state Cox depended only on D, while steady-state biomass concentration was controlled by both D and K,a. Given excess oxygen, cells grew subject to Monod limitation by xylose, which became inhibitory above 40 g/L. Ethanol inhibition was consistent with Luong's model, and 64.3 g/L was the maximum ethanol concentration allowing growth. Actively growing cells died at a rate that was 20% of micro. The dying portion increased with E and X
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260371012