A sensitive immunosensor using colloidal gold as electrochemical label

A sensitive immunosensor using colloidal gold as electrochemical label is described. In this method, the capture protein was first immobilized on a carbon paste electrode surface through passive adsorption to bind quantitatively with corresponding antigen and colloidal gold labeled antibody to perfo...

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Bibliographic Details
Published in:Talanta (Oxford) 2007-07, Vol.72 (5), p.1800-1804
Main Authors: Chen, Zhao-Peng, Peng, Zhao-Feng, Zhang, Peng, Jin, Xue-Fang, Jiang, Jian-Hui, Zhang, Xiao-Bing, Shen, Guo-Li, Yu, Ru-Qin
Format: Article
Language:English
Subjects:
Adsorptive voltammetry
Analytical chemistry
Chemistry
Colloidal gold
Electrochemical label
Electrochemical methods
Exact sciences and technology
General, instrumentation
Immunosensor
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Summary:A sensitive immunosensor using colloidal gold as electrochemical label is described. In this method, the capture protein was first immobilized on a carbon paste electrode surface through passive adsorption to bind quantitatively with corresponding antigen and colloidal gold labeled antibody to perform a sandwich assay. To detect the amount of the colloidal gold captured on the electrode surface, the colloid was first oxidized electrochemically to produce AuCl 4 − ions which were adsorbed strongly on the electrode surface. Adsorptive voltammetry was then employed for the determination of the adsorbed AuCl 4 − ions. A linear relationship between reduction wave peak current and the antigen concentration (human IgG) from 10 to 500 ng/ml is obtained with a detection limit of 4.0 ng/ml.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2007.02.020