Loading…

Application and detection of (14)c-hd in two mouse models

The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology mechanisms and methods 2002, Vol.12 (4), p.293-300
Main Authors: Logan, Thomas P, Shutz, Michael, Schulz, Susan M, Railer, Roy, Ricketts, Karen M, Casillas, Robert P
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 300
container_issue 4
container_start_page 293
container_title Toxicology mechanisms and methods
container_volume 12
creator Logan, Thomas P
Shutz, Michael
Schulz, Susan M
Railer, Roy
Ricketts, Karen M
Casillas, Robert P
description The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the (14)C content in the skin after exposures to HD containing (14)C-HD. Rate curves were generated for applications of (1) HD in methylene chloride to the haired mouse ear; (2) HD in methylene chloride to the hairless mouse dorsal skin; and (3) saturated HD vapor to the hairless mouse dorsal skin for 6 min. The curves showed a reduction in (14)C disintegrations per min in animals euthanized 0 to 2 h postexposure. The largest percentage of decrease of (14)C content in skin occurred within 30 min of HD challenge for all exposures. An 8-mm skin-punch biopsy and a 14-mm annular skin section surrounding the region of the 8-mm skin punch were taken from the hairless mouse dorsal skin exposed to HD in methylene chloride. The ratio of the (14)C content in the 8-mm skin punch to that in the surrounding 14-mm annular skin section was 7.3, demonstrating that the HD application spreads beyond the initially biopsied site. A concentration/time value of 6.3 mug/cm(2)/min was determined by counting skin (14)C disintegrations per minute in animals euthanized immediately after exposure to saturated HD vapor. Determinations of the amount of HD showed that similar quantities of HD, 0.4 mg, were detected on each model. These results contribute to a better quantitative understanding of HD application in the haired and hairless mouse models.
doi_str_mv 10.1080/15376520208951165
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_734202218</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>734202218</sourcerecordid><originalsourceid>FETCH-LOGICAL-p550-2b213893d57647881159f0d078ce4ee94b4cbbcdf000f46ef668fc3e91208dad3</originalsourceid><addsrcrecordid>eNo1j0tLxEAQhAdB3HX1B3iRuamHaPe8c1yW9QELXvYekpkejORlJov47426XrrooijqY-wK4R7BwQNqaY0WIMDlGtHoE7b88bLZVAt2ntI7ADpUeMYWAkAgWliyfD0MTe3Lqe47XnaBB5rI_3595Leo7nz2Fnjd8emz521_SDTfQE26YKexbBJdHnXF9o_b_eY5270-vWzWu2zQGjJRCZQul0Fbo6xziDqPEMA6T4ooV5XyVeVDBICoDEVjXPSScpxBQhnkit381Q5j_3GgNBVtnTw1TdnRvKawUs3QAt2cvD4mD1VLoRjGui3Hr-IfVn4DUaFSRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734202218</pqid></control><display><type>article</type><title>Application and detection of (14)c-hd in two mouse models</title><source>Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list)</source><creator>Logan, Thomas P ; Shutz, Michael ; Schulz, Susan M ; Railer, Roy ; Ricketts, Karen M ; Casillas, Robert P</creator><creatorcontrib>Logan, Thomas P ; Shutz, Michael ; Schulz, Susan M ; Railer, Roy ; Ricketts, Karen M ; Casillas, Robert P</creatorcontrib><description>The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the (14)C content in the skin after exposures to HD containing (14)C-HD. Rate curves were generated for applications of (1) HD in methylene chloride to the haired mouse ear; (2) HD in methylene chloride to the hairless mouse dorsal skin; and (3) saturated HD vapor to the hairless mouse dorsal skin for 6 min. The curves showed a reduction in (14)C disintegrations per min in animals euthanized 0 to 2 h postexposure. The largest percentage of decrease of (14)C content in skin occurred within 30 min of HD challenge for all exposures. An 8-mm skin-punch biopsy and a 14-mm annular skin section surrounding the region of the 8-mm skin punch were taken from the hairless mouse dorsal skin exposed to HD in methylene chloride. The ratio of the (14)C content in the 8-mm skin punch to that in the surrounding 14-mm annular skin section was 7.3, demonstrating that the HD application spreads beyond the initially biopsied site. A concentration/time value of 6.3 mug/cm(2)/min was determined by counting skin (14)C disintegrations per minute in animals euthanized immediately after exposure to saturated HD vapor. Determinations of the amount of HD showed that similar quantities of HD, 0.4 mg, were detected on each model. These results contribute to a better quantitative understanding of HD application in the haired and hairless mouse models.</description><identifier>EISSN: 1537-6524</identifier><identifier>DOI: 10.1080/15376520208951165</identifier><identifier>PMID: 20021170</identifier><language>eng</language><publisher>England</publisher><ispartof>Toxicology mechanisms and methods, 2002, Vol.12 (4), p.293-300</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20021170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Logan, Thomas P</creatorcontrib><creatorcontrib>Shutz, Michael</creatorcontrib><creatorcontrib>Schulz, Susan M</creatorcontrib><creatorcontrib>Railer, Roy</creatorcontrib><creatorcontrib>Ricketts, Karen M</creatorcontrib><creatorcontrib>Casillas, Robert P</creatorcontrib><title>Application and detection of (14)c-hd in two mouse models</title><title>Toxicology mechanisms and methods</title><addtitle>Toxicol Mech Methods</addtitle><description>The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the (14)C content in the skin after exposures to HD containing (14)C-HD. Rate curves were generated for applications of (1) HD in methylene chloride to the haired mouse ear; (2) HD in methylene chloride to the hairless mouse dorsal skin; and (3) saturated HD vapor to the hairless mouse dorsal skin for 6 min. The curves showed a reduction in (14)C disintegrations per min in animals euthanized 0 to 2 h postexposure. The largest percentage of decrease of (14)C content in skin occurred within 30 min of HD challenge for all exposures. An 8-mm skin-punch biopsy and a 14-mm annular skin section surrounding the region of the 8-mm skin punch were taken from the hairless mouse dorsal skin exposed to HD in methylene chloride. The ratio of the (14)C content in the 8-mm skin punch to that in the surrounding 14-mm annular skin section was 7.3, demonstrating that the HD application spreads beyond the initially biopsied site. A concentration/time value of 6.3 mug/cm(2)/min was determined by counting skin (14)C disintegrations per minute in animals euthanized immediately after exposure to saturated HD vapor. Determinations of the amount of HD showed that similar quantities of HD, 0.4 mg, were detected on each model. These results contribute to a better quantitative understanding of HD application in the haired and hairless mouse models.</description><issn>1537-6524</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNo1j0tLxEAQhAdB3HX1B3iRuamHaPe8c1yW9QELXvYekpkejORlJov47426XrrooijqY-wK4R7BwQNqaY0WIMDlGtHoE7b88bLZVAt2ntI7ADpUeMYWAkAgWliyfD0MTe3Lqe47XnaBB5rI_3595Leo7nz2Fnjd8emz521_SDTfQE26YKexbBJdHnXF9o_b_eY5270-vWzWu2zQGjJRCZQul0Fbo6xziDqPEMA6T4ooV5XyVeVDBICoDEVjXPSScpxBQhnkit381Q5j_3GgNBVtnTw1TdnRvKawUs3QAt2cvD4mD1VLoRjGui3Hr-IfVn4DUaFSRg</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Logan, Thomas P</creator><creator>Shutz, Michael</creator><creator>Schulz, Susan M</creator><creator>Railer, Roy</creator><creator>Ricketts, Karen M</creator><creator>Casillas, Robert P</creator><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Application and detection of (14)c-hd in two mouse models</title><author>Logan, Thomas P ; Shutz, Michael ; Schulz, Susan M ; Railer, Roy ; Ricketts, Karen M ; Casillas, Robert P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p550-2b213893d57647881159f0d078ce4ee94b4cbbcdf000f46ef668fc3e91208dad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Logan, Thomas P</creatorcontrib><creatorcontrib>Shutz, Michael</creatorcontrib><creatorcontrib>Schulz, Susan M</creatorcontrib><creatorcontrib>Railer, Roy</creatorcontrib><creatorcontrib>Ricketts, Karen M</creatorcontrib><creatorcontrib>Casillas, Robert P</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology mechanisms and methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Logan, Thomas P</au><au>Shutz, Michael</au><au>Schulz, Susan M</au><au>Railer, Roy</au><au>Ricketts, Karen M</au><au>Casillas, Robert P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application and detection of (14)c-hd in two mouse models</atitle><jtitle>Toxicology mechanisms and methods</jtitle><addtitle>Toxicol Mech Methods</addtitle><date>2002</date><risdate>2002</risdate><volume>12</volume><issue>4</issue><spage>293</spage><epage>300</epage><pages>293-300</pages><eissn>1537-6524</eissn><abstract>The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the (14)C content in the skin after exposures to HD containing (14)C-HD. Rate curves were generated for applications of (1) HD in methylene chloride to the haired mouse ear; (2) HD in methylene chloride to the hairless mouse dorsal skin; and (3) saturated HD vapor to the hairless mouse dorsal skin for 6 min. The curves showed a reduction in (14)C disintegrations per min in animals euthanized 0 to 2 h postexposure. The largest percentage of decrease of (14)C content in skin occurred within 30 min of HD challenge for all exposures. An 8-mm skin-punch biopsy and a 14-mm annular skin section surrounding the region of the 8-mm skin punch were taken from the hairless mouse dorsal skin exposed to HD in methylene chloride. The ratio of the (14)C content in the 8-mm skin punch to that in the surrounding 14-mm annular skin section was 7.3, demonstrating that the HD application spreads beyond the initially biopsied site. A concentration/time value of 6.3 mug/cm(2)/min was determined by counting skin (14)C disintegrations per minute in animals euthanized immediately after exposure to saturated HD vapor. Determinations of the amount of HD showed that similar quantities of HD, 0.4 mg, were detected on each model. These results contribute to a better quantitative understanding of HD application in the haired and hairless mouse models.</abstract><cop>England</cop><pmid>20021170</pmid><doi>10.1080/15376520208951165</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier EISSN: 1537-6524
ispartof Toxicology mechanisms and methods, 2002, Vol.12 (4), p.293-300
issn 1537-6524
language eng
recordid cdi_proquest_miscellaneous_734202218
source Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list)
title Application and detection of (14)c-hd in two mouse models
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T11%3A54%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Application%20and%20detection%20of%20(14)c-hd%20in%20two%20mouse%20models&rft.jtitle=Toxicology%20mechanisms%20and%20methods&rft.au=Logan,%20Thomas%20P&rft.date=2002&rft.volume=12&rft.issue=4&rft.spage=293&rft.epage=300&rft.pages=293-300&rft.eissn=1537-6524&rft_id=info:doi/10.1080/15376520208951165&rft_dat=%3Cproquest_pubme%3E734202218%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p550-2b213893d57647881159f0d078ce4ee94b4cbbcdf000f46ef668fc3e91208dad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=734202218&rft_id=info:pmid/20021170&rfr_iscdi=true