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Biosynthesis of a Fully Functional Cyclotide inside Living Bacterial Cells

Perfect circle. We report the biosynthesis of a natively folded cyclotide, MCoTI‐II, in E. coli by intracellular backbone cyclization of a linear cyclotide–intein fusion precursor. The cyclized peptide then spontaneously folds into its native conformation. Biosynthetic access to correctly folded cyc...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2007-08, Vol.8 (12), p.1363-1366
Main Authors: Camarero, Julio A, Kimura, Richard H, Woo, Youn-Hi, Shekhtman, Alexander, Cantor, Jason
Format: Article
Language:English
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Summary:Perfect circle. We report the biosynthesis of a natively folded cyclotide, MCoTI‐II, in E. coli by intracellular backbone cyclization of a linear cyclotide–intein fusion precursor. The cyclized peptide then spontaneously folds into its native conformation. Biosynthetic access to correctly folded cyclotides allows the possibility of generating cell‐based combinatorial libraries that can be screened, inside living cells, for their ability to modulate or inhibit cellular processes.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200700183